A Combined microRNA and Chemokine Profile in Urine to Identify Rejection After Kidney Transplantation.

ABSTRACT: There is an unmet need for noninvasive tools for diagnosis of rejection after kidney transplantation. The aim of this study was to determine the discriminative value of a combined cellular and molecular biomarker platform in urine for the detection of rejection.

Methods

First, microRNA (miR) molecules were screened in transplant biopsies and urine sediments of patients with acute rejection and patients without rejection and stable graft function. Second, the expression of 15 selected miRs was quantified in an independent set of 115 urine sediments of patients with rejection and 55 urine sediments of patients without histological signs of rejection on protocol biopsy. Additionally, CXCL-9 and CXCL-10 protein levels were quantified in the urine supernatant.

Results

Levels of mi

SUBMITTER: Gielis EM 

PROVIDER: S-EPMC8196093 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature