Cell-type-specific, multicolor labeling of endogenous proteins with split fluorescent protein tags in Drosophila.

ABSTRACT: The impact of the Drosophila experimental system on studies of modern biology cannot be understated. The ability to tag endogenously expressed proteins is essential to maximize the use of this model organism. Here, we describe a method for labeling endogenous proteins with self-complementing split fluorescent proteins (split FPs) in a cell-type-specific manner in Drosophila A short fragment of an FP coding sequence is inserted into a specific genomic locus while the remainder of the FP is expressed using an available GAL4 driver line. In consequence, complementation fluorescence allows examination of protein localization in particular cells. Besides, when inserting tandem repeats of the short FP fragment at the same genomic locus, we can substantially enhance the fluor

SUBMITTER: Kamiyama R 

PROVIDER: S-EPMC8201798 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature