Genomic data reveals the emergence of an IncQ1 small plasmid carrying bla_KPC-2 in Escherichia coli of the pandemic sequence type 648.

ABSTRACT:

Objectives

The global success of carbapenem-resistant pathogens has been attributed to large plasmids carrying bla_KPC genes circulating among high-risk clones. In this study, we sequenced the genome of a carbapenem-resistant Escherichia coli strain (Ec351) isolated from a human infection. Phylogenomic analysis based on single nucleotide polymorphisms (SNPs) as well as the comparative resistome and plasmidome of globally disseminated bla_KPC-2-positive E. coli strains with identical sequence type (ST) were further investigated.

Methods

Total DNA was sequenced using an Illumina NextSeq 500 platform and was assembled using Unicycler. Genomic data were evaluated through bioinformatics tools available from the Center of Genomic Epidemiology and by in silico a

SUBMITTER: Fuga B 

PROVIDER: S-EPMC8213540 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature