Calcium enhances polyplex-mediated transfection efficiency of plasmid DNA in Jurkat cells.

ABSTRACT: Jurkat, an immortalized cell line derived from human leukemic T lymphocytes, has been employed as an excellent surrogate model of human primary T-cells for the advancement of T-cell biology and their applications in medicine. However, presumably due to its T-cell origin, Jurkat cells are very difficult to transfect. Thus, for the genetic modification of Jurkat cells, expensive and time-consuming viral vectors are normally required. Despite many previous efforts, non-viral vectors have not yet overcome the hurdles of low transfection efficiency and/or high toxicity in transfection of Jurkat cells. Here, we report that a simple addition of calcium ions (Ca²⁺) into culture media at optimal concentrations can enhance the efficiency of the polyplex-mediated transfection using poly(et

SUBMITTER: Ayyadevara VSSA 

PROVIDER: S-EPMC8216448 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature