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Effect of colloid-size copper-based pesticides and wood-preservatives against microbial activities of Gram-positive Bacillus species using five-day biochemical oxygen demand test.

ABSTRACT: Copper-based pesticides and wood preservatives could end up in the environment during production, use, and end-of-life via different pathways that could cause unintended ecological and adverse health effects. This paper provides the effect of colloid-size Cu-based pesticides (CuPRO and Kocide), micronized Cu azole (MCA-1 and MCA-2) and alkaline Cu quaternary (ACQ) treated woods, Cu²⁺, Cu²⁺ spiked untreated wood (UTW), and CuCO₃ solutions against Gram-positive Bacillus species using five-day biochemical oxygen demand (BOD₅) standard test. The total Cu leached from MCA-1, MCA-2, and ACQ in Milli-Q water after 5 days were ~0.1, ~0.11, and ~0.64 g/kg of wood, respectively. However, the form of Cu leached from MCA woods was mostly ionic (> 90%). The total organic carbon (TOC) content of any tested wood (UTW/MCA-1/MCA-2/ACQ) was ~99% of its corresponding total carbon (TC) content, whereas the TOC of any tested wood sawdust exceeded that of its corresponding piece/block by > 300%. The dissolved oxygen (DO) consumption value in the presence of Cu²⁺, CuCO₃, CuPRO, and Kocide solutions was significantly influenced by Cu particles/ions. However, the DO consumption value in the presence of UTW/MCA-1/MCA-2/ACQ woods was significantly influenced by organics leached from woods. On the other hand, the DO consumption of MCA sawdust was greater than (300%) that of MCA pieces/block. The findings of this study provide more insight into how organics leached from woods significantly reduce the toxic effects of Cu ions against Gram-positive Bacillus species.

SUBMITTER: Tegenaw A

PROVIDER: S-EPMC8217730 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Project description:Copper-based fungicides are routinely used for wood and plant protection, which can lead to an enrichment of copper-tolerant microbial communities in soil. To investigate the effect of such wood preservatives on the soil fungal and bacterial community compositions, five different vineyard and fruit-growing soil environments were evaluated using incubation studies over time. Pine sapwood specimens were impregnated with either water or different biocide treatment solutions containing a mixture of copper, triazoles, and quaternary ammonium compounds (CuTriQAC), a mixture of triazoles and quaternary ammonium compounds (TriQAC), or copper alone (Cu). Specimens were incubated in soil from each sample site for 8, 16, 24, and 32 weeks. The effects of preservative treatment on the modulus of elasticity (MOE) of the wood specimens and on the soil fungal as well as bacterial community composition at the soil-wood interface were assessed by quantitative PCR and amplicon sequencing of the fungal internal transcribed spacer (ITS) region and bacterial 16S rRNA gene. Specimens impregnated with CuTriQAC and Cu showed decreased MOE and reduced fungal and bacterial copy numbers over time compared to those impregnated with water and TriQAC. Fungal but not bacterial community composition was significantly affected by wood preservative treatment. The relative abundance of members of the family Trichocomaceae compared to other genera increased in the presence of the Cu and CuTriQAC treatments at three sites, suggesting these to be Cu-tolerant fungi. In conclusion, the copper-containing treatments resulted in marginally increased MOE, lowered microbial gene copy numbers compared to those in the TriQAC and water treatments, and thus enhanced wood protection against soil microbial wood degradation.IMPORTANCE Copper-containing rather than TRIQAC formulations are efficient wood preservatives irrespective of the origin and composition of the soil microbial communities. However, some fungi appear to be naturally insensitive to copper and should be the focus of future wood preservative formulations to facilitate the life span of wooden construction in contact with soil while also minimizing the overall environmental impact.

Project description:Following the futile efforts of generations to reach the high standard of excellence achieved by the luthiers in Cremona, Italy, by variations of design and plate tuning, current interest is being focused on differences in material properties. The long-standing question whether the wood of Stradivari and Guarneri were treated with wood preservative materials could be answered only by the examination of wood specimens from the precious antique instruments. In a recent communication (Nature, 2006), we reported about the degradation of the wood polymers in instruments of Stradivari and Guarneri, which could be explained only by chemical manipulations, possibly by preservatives. The aim of the current work was to identify the minerals from the small samples of the maple wood which were available to us from the antique instruments. The ashes of wood from one violin and one cello by Stradivari, two violins by Guarneri, one viola by H. Jay, one violin by Gand-Bernardel were analyzed and compared with a variety of commercial tone woods. The methods of analysis were the following: back-scattered electron imaging, X-ray fluorescence maps for individual elements, wave-length dispersive spectroscopy, energy dispersive X-ray spectroscopy and quantitative microprobe analysis. All four Cremonese instruments showed the unmistakable signs of chemical treatments in the form of chemicals which are not present in natural woods, such as BaSO4, CaF2, borate, and ZrSiO4. In addition to these, there were also changes in the common wood minerals. Statistical evaluation of 12 minerals by discriminant analysis revealed: a. a difference among all four Cremona instruments, b. the difference of the Cremonese instruments from the French and English antiques, and c. only the Cremonese instruments differed from all commercial woods. These findings may provide the answer why all attempts to recreate the Stradivarius from natural wood have failed. There are many obvious implications with regard to how the green tone wood should be treated, which chould lead to changes in the practice of violin-making. This research should inspire others to analyze more antique violins for their chemical contents.

Project description:Heartwood extracts of naturally durable wood species are often evaluated as alternatives to chemical wood preservatives, but field data from long-term performance testing are lacking. The current study evaluated the long-term (five-year) performance of two non-durable wood species treated with heartwood extracts of either Tectona grandis, Dalbergia sissoo, Cedrus deodara, or Pinus roxburghii alone or combined with linseed oil. Stakes (45.7 × 1.9 × 1.9 cm) and blocks (12.5 × 3.75 × 2.5 cm) cut from the sapwood of cottonwood and southern pine were vacuum-pressure impregnated with the individual heartwood species extract, linseed oil, or a mixture of each individual wood extract and linseed oil. For comparison, solid heartwood stakes and blocks of the wood species used to obtain extracts were also included in the tests. All samples were exposed for five years to decay and termites at a test site in southern Mississippi using ground contact (AWPA E7) and ground proximity (AWPA E26) tests. Results showed that extract-oil mixtures imparted higher termite and decay resistance in cottonwood and southern pine than linseed oil only or the individual heartwood species extract in both tests. However, these treatments were as not effective as to commercially used wood preservatives, copper naphthenate (CuN) or disodium octaborate tetrahydrate (DOT) in either test. Moreover, solid heartwood P. roxburghii stakes were completely decayed and attacked by termites after five years in the ground contact test. In contrast, C. deodara stakes were slightly attacked by termites and moderately attacked by decay fungi. However, T. grandis and D. sissoo stakes showed slight to superficial attack by termites and decay fungi in ground contact test. In contrast, T. grandis and D. sissoo blocks showed slight decay fungi attack in above-ground tests. However, termites did not attack T. grandis, D. sissoo, and C. deodara blocks. However, decay fungi moderately attacked C. deodara blocks, and P. roxburghii blocks were severely attacked by decay fungi and termites in the above-ground test.

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Effect of colloid-size copper-based pesticides and wood-preservatives against microbial activities of Gram-positive Bacillus species using five-day biochemical oxygen demand test.

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