Three-dimensional cultured mesenchymal stem cells enhance repair of ischemic stroke through inhibition of microglia.

ABSTRACT:

Background

We show previously that three-dimensional (3D) spheroid cultured mesenchymal stem cells (MSCs) exhibit reduced cell size thus devoid of lung entrapment following intravenous (IV) infusion. In this study, we determined the therapeutic effect of 3D-cultured MSCs on ischemic stroke and investigated the mechanisms involved.

Methods

Rats underwent middle cerebral artery occlusion (MCAO) and reperfusion. 1 × 10⁶ of 3D- or 2D-cultured MSCs, which were pre-labeled with GFP, were injected through the tail vain three and seven days after MCAO. Two days after infusion, MSC engraftment into the ischemic brain tissues was assessed by histological analysis for GFP-expressing cells, and infarct volume was determined by MRI. Microglia in the lesion were sorted and subj

SUBMITTER: Li Y 

PROVIDER: S-EPMC8218508 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature