Ontology highlight
ABSTRACT:
SUBMITTER: Yao R
PROVIDER: S-EPMC8219879 | biostudies-literature | 2021
REPOSITORIES: biostudies-literature
Frontiers in veterinary science 20210609
Bovine Viral Diarrhea Virus (BVDV) is the main pathogen of bovine viral diarrhea disease (BVD), which leads to enormous economic losses in the cattle industry. A sensitive and specific detection for BVDV is advantageous to the control of BVDV. Clustered regularly interspaced short palindromic repeats (CRISPR)-Cas systems have been used for detecting virus RNA. In this study, the expression and purification of LwCas13a protein was optimized and the RNase activity of LwCas13a <i>in vitro</i> was v ...[more]