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Generation of interspecies mouse-rat chimeric embryos by embryonic stem (ES) cell microinjection.

ABSTRACT: Interspecies chimerism is a useful tool to study interactions between cells of different genetic makeup in order to elucidate the mechanisms underlying non-cell-autonomous processes, including evolutionary events. However, generating interspecies chimeras with high efficiency and chimerism level remains challenging. Here, we describe a protocol for generating chimeras between mouse and rat. Donor embryonic stem cells of one species are microinjected into early embryos of the other species (recipient), which are implanted into host foster mothers of the recipient species. For complete details on the use and execution of this protocol, please refer to Stepien et al. (2020).

SUBMITTER: Stepien BK

PROVIDER: S-EPMC8219896 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature

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Generation of interspecies mouse-rat chimeric embryos by embryonic stem (ES) cell microinjection.

Stepien Barbara K BK Vaid Samir S Naumann Ronald R Holtz Anja A Huttner Wieland B WB

STAR protocols 20210615 2

Interspecies chimerism is a useful tool to study interactions between cells of different genetic makeup in order to elucidate the mechanisms underlying non-cell-autonomous processes, including evolutionary events. However, generating interspecies chimeras with high efficiency and chimerism level remains challenging. Here, we describe a protocol for generating chimeras between mouse and rat. Donor embryonic stem cells of one species are microinjected into early embryos of the other species (recip ...[more]

PMID: 34189467

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