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Clustering of Death Receptor for Apoptosis Using Nanoscale Patterns of Peptides.

ABSTRACT: The nanoscale spatial organization of transmembrane tumor necrosis factor (TNF) receptors has been implicated in the regulation of cellular fate. Accordingly, molecular tools that can induce specific arrangements of these receptors on cell surfaces would give us an opportunity to study these effects in detail. To achieve this, we introduce DNA origami nanostructures that precisely scaffold the patterning of TNF-related apoptosis-inducing ligand-mimicking peptides at nanoscale level. Stimulating human breast cancer cells with these patterns, we find that around 5 nm is the critical interligand distance of hexagonally patterned peptides to induce death receptor clustering and a resulting apoptosis. We thus offer a strategy to reverse the non-efficacy of current ligand- and antibody-based methods for TNF superfamily activation.

SUBMITTER: Wang Y

PROVIDER: S-EPMC8223489 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature

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Clustering of Death Receptor for Apoptosis Using Nanoscale Patterns of Peptides.

Wang Yang Y Baars Igor I Fördös Ferenc F Högberg Björn B

ACS nano 20210521 6

The nanoscale spatial organization of transmembrane tumor necrosis factor (TNF) receptors has been implicated in the regulation of cellular fate. Accordingly, molecular tools that can induce specific arrangements of these receptors on cell surfaces would give us an opportunity to study these effects in detail. To achieve this, we introduce DNA origami nanostructures that precisely scaffold the patterning of TNF-related apoptosis-inducing ligand-mimicking peptides at nanoscale level. Stimulating ...[more]

PMID: 34019379

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