Ontology highlight
ABSTRACT:
SUBMITTER: Kosar M
PROVIDER: S-EPMC8225803 | biostudies-literature | 2021 Jun
REPOSITORIES: biostudies-literature
Kosar Martin M Giannattasio Michele M Piccini Daniele D Maya-Mendoza Apolinar A García-Benítez Francisco F Bartkova Jirina J Barroso Sonia I SI Gaillard Hélène H Martini Emanuele E Restuccia Umberto U Ramirez-Otero Miguel Angel MA Garre Massimiliano M Verga Eleonora E Andújar-Sánchez Miguel M Maynard Scott S Hodny Zdenek Z Costanzo Vincenzo V Kumar Amit A Bachi Angela A Aguilera Andrés A Bartek Jiri J Foiani Marco M
Nature communications 20210624 1
Although human nucleoporin Tpr is frequently deregulated in cancer, its roles are poorly understood. Here we show that Tpr depletion generates transcription-dependent replication stress, DNA breaks, and genomic instability. DNA fiber assays and electron microscopy visualization of replication intermediates show that Tpr deficient cells exhibit slow and asymmetric replication forks under replication stress. Tpr deficiency evokes enhanced levels of DNA-RNA hybrids. Additionally, complementary prot ...[more]