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The Antioxidant Activity of Limonene Counteracts Neurotoxicity Triggered byAβ_1-42 Oligomers in Primary Cortical Neurons.

ABSTRACT: Many natural-derived compounds, including the essential oils from plants, are investigated to find new potential protective agents in several neurodegenerative disorders such as Alzheimer's disease (AD). In the present study, we tested the neuroprotective effect of limonene, one of the main components of the genus Citrus, against the neurotoxicity elicited by Aβ_1-42 oligomers, currently considered a triggering factor in AD. To this aim, we assessed the acetylcholinesterase activity by Ellman's colorimetric method, the mitochondrial dehydrogenase activity by MTT assay, the nuclear morphology by Hoechst 33258, the generation of reactive oxygen species (ROS) by DCFH-DA fluorescent dye, and the electrophysiological activity of K_V3.4 potassium channel subunits by patch-clamp electrophysiology. Interestingly, the monoterpene limonene showed a specific activity against acetylcholinesterase with an IC₅₀ almost comparable to that of galantamine, used as positive control. Moreover, at the concentration of 10 µg/mL, limonene counteracted the increase of ROS production triggered by Aβ_1-42 oligomers, thus preventing the upregulation of K_V3.4 activity. This, in turn, prevented cell death in primary cortical neurons, showing an interesting neuroprotective profile against Aβ_1-42-induced toxicity. Collectively, the present results showed that the antioxidant properties of the main component of the genus Citrus, limonene, may be useful to prevent neuronal suffering induced by Aβ_1-42 oligomers preventing the hyperactivity of K_V3.4.

SUBMITTER: Piccialli I

PROVIDER: S-EPMC8227170 | biostudies-literature |

REPOSITORIES: biostudies-literature

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The Antioxidant Activity of Limonene Counteracts Neurotoxicity Triggered byAβ_1-42 Oligomers in Primary Cortical Neurons.

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The Antioxidant Activity of Limonene Counteracts Neurotoxicity Triggered byAβ1-42 Oligomers in Primary Cortical Neurons.

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OmicsDI is part of the ELIXIR infrastructure

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The Antioxidant Activity of Limonene Counteracts Neurotoxicity Triggered byAβ_1-42 Oligomers in Primary Cortical Neurons.