Inherent and Stress-Induced Responses of Fine Root Morphology and Anatomy in Commercial Grapevine Rootstocks with Contrasting Drought Resistance.
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ABSTRACT:
SUBMITTER: Reingwirtz I
PROVIDER: S-EPMC8227383 | biostudies-literature |
REPOSITORIES: biostudies-literature
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Project description:BACKGROUND:Roots play a central role in plant response to water stress (WS). They are involved in its perception and signalling to the leaf as well as in allowing the plant to adapt to maintaining an adequate water balance. Only a few studies have investigated the molecular/biochemical responses to WS in roots of perennial plants, such as grapevine. This study compares two grapevine rootstock genotypes (i.e. 101.14 and M4) with different tolerance to WS, evaluating the responses at proteomic and metabolite levels. RESULTS:WS induced changes in the abundance of several proteins in both genotypes (17 and 22% of the detected proteins in 101.14 and M4, respectively). The proteomic analysis revealed changes in many metabolic pathways that fitted well with the metabolite data. M4 showed metabolic responses which were potentially able to counteract the WS effects, such as the drop in cell turgor, increased oxidative stress and loss of cell structure integrity/functionality. However, in 101.14 it was evident that the roots were suffering more severely from these effects. We found that many proteins classified as active in energy metabolism, hormone metabolism, protein, secondary metabolism and stress functional classes showed particular differences between the two rootstocks. CONCLUSION:The proteomic/metabolite comparative analysis carried out provides new information on the possible biochemical and molecular strategies adopted by grapevine roots to counteract WS. Although further work is needed to define in detail the role(s) of the proteins and metabolites that characterize WS response, this study, involving the M4 rootstock genotype, highlights that osmotic responses, modulations of C metabolism, mitochondrial functionality and some specific responses to stress occurring in the roots play a primary role in Vitis spp. tolerance to this type of abiotic stress.
| S-EPMC6011575 | biostudies-literature
Project description:Water deficits are known to alter fine root structure and function, but little is known about how these responses contribute to differences in drought resistance across grapevine rootstocks. The ways in which water deficit affects root anatomical and physiological characteristics were studied in two grapevine rootstocks considered as low-medium (101-14Mgt) and highly (110R) drought resistant. Rootstocks were grown under prolonged and repeated drying cycles or frequent watering ('dry' and 'wet' treatments, respectively), and the following parameters were evaluated: root osmotic and hydrostatic hydraulic conductivity (Lp os and Lp hyd, respectively), suberization, steady-state root pressure (P rs), sap exudation rates, sap osmotic potential, and exosmotic relaxation curves. For both rootstocks, the 'dry' treatment reduced fine root Lp, elicited earlier root suberization and higher sap osmotic potential, and generated greater P rs after rewatering, but the rootstocks responded differently under these conditions. Lp os, Lp hyd, and sap exudation rates were significantly higher in 110R than in 101-14Mgt, regardless of moisture treatment. Under 'dry' conditions, 110R maintained a similar Lp os and decreased the Lp hyd by 36% compared with 'wet' conditions, while both parameters were decreased by at least 50% for 101-14Mgt under 'dry' conditions. Interestingly, build-up of P rs in 110R was 34% lower on average than in 101-14Mgt, suggesting differences in the development of suberized apoplastic barriers between the rootstocks as visualized by analysis of suberization from fluorescence microscopy. Consistent with this pattern, 110R exhibited the greatest exosmotic Lp os (i.e. Lp os of water flowing from roots to the soil) as determined from relaxation curves under wet conditions, where backflow may have limited its capacity to generate positive xylem pressure. The traits studied here can be used in combination to provide new insights needed for screening drought resistance across grapevine rootstocks.
| S-EPMC4615816 | biostudies-other
Project description:Trees adjust multiple structural and functional organ-specific characteristics, "traits", to cope with diverse soil conditions. Studies on traits are widely used to uncover ecological species adaptability to varying environments. However, fine-root traits are rarely studied for methodological reasons. We analyzed the adaptability of the fine-root systems of European beech and Norway spruce to extreme drought within species-specific tree groups at Kranzberger Forst (Germany), focusing on the seasonality of morphological, physiological, and biochemical key traits in view of carbon (C) and nitrogen dynamics. We hypothesized that fine roots of both species adjust to seasonal drought: with beech representing a "fast" (i.e. with fast C turnover), and spruce a "slow" (i.e. with long-term C retention) ecological strategy. We identified three functional fine-root categories, based on root function (absorptive or transport fine roots), and mycorrhizal status of the absorptive fine-roots (mycorrhizal or non-mycorrhizal). Solely the non-mycorrhizal absorptive roots adjusted in a species-specific manner supporting fine-root ecological strategy hypothesis. During drought, beech produced thin ephemeral (absorptive non-mycorrhizal) fine roots with high specific fine-root area and high respiratory activity, representing fast C turnover and enabling effective resource exploitation. These adjustments reflect a "fast" ecological strategy. Conversely, spruce absorptive fine roots did not respond to the soil moisture deficit by growth but instead increased root suberization. Drastically lowered respiratory activity of this functional category facilitated C retention and structural persistence during drought, indicating a "slow" ecological strategy in spruce. Absorptive mycorrhizal fine roots maintained respiration throughout the drought event in both tree species, but in spruce this was the only fine-root category with high respiration. This suggests, that spruce relies heavily on mycorrhizal associations as a method of drought resistance. Accumulation of non-structural carbohydrates and high C concentrations were observed in the transport fine roots of both species, indicating drought-induced osmotic protection of these roots. Thus, functional classification enabled us to determine that fine-root branches of each species are not tied to one sole ecological strategy. The suggested approach helps to better understand the complex interplay between structure and function belowground.
| S-EPMC7438540 | biostudies-literature
Project description:Drought is one of the main factors affecting sweet cherry yields, and cherry rootstocks can provide a range of tree vigor levels to better match sweet cherries with the characteristics of the soil. To investigate the molecular events of the cherry to water deficiency, we performed transcriptomic and metabolomic analyses of Prunus mahaleb CDR-1 (drought-tolerant cherry rootstock (DT)) and P. cerasus × P. canescens Gisela 5 (drought-susceptible cherry rootstock (DS)), respectively. The results revealed 253 common drought-responsive genes in leaves and roots in DT and 17 in DS; 59 upregulated metabolites were explored in leaves in DT and 19 were explored in DS. Differentially expressed metabolites related to the cyanoamino acid metabolism pathway and phenylpropanoid biosynthesis pathway may be key factors in the difference in drought resistance in the two rootstocks. Moreover, six central metabolites-3-cyanoalanine, phenylalanine, quinic acid, asparagine, p-benzoquinone, and phytosphingosine-were identified as potential biological markers of drought response in cherries and may be key factors in the difference in drought resistance, along with caffeic acid and chlorogenic acid. We also selected 17 differentially expressed genes as core candidate genes and the mechanism of DT in response to drought is summarized.
| S-EPMC8001747 | biostudies-literature
Project description:Phosphorus (P) is an important macronutrient for all lives, but it is also a finite resource. Therefore, it is important to understand how to increase the P availability and plant uptake. The endophytes can help host plants to improve P uptake and will be apparently affected by plant genotypes. To investigate the mechanism of root endophytes in promoting P uptake of peach rootstocks, we analyzed the variations of the root endophytic fungal and bacterial communities of peach rootstocks with different P efficiencies under high or low level of P addition. Results showed that Proteobacteria was the dominant bacterial phylum in the roots of all rootstocks under the two levels of P addition. At low P level, the abundance of Actinoplanes in phosphorus-inefficiency root system was apparently higher than that at high P level. Actinoplanes produced important secondary metabolites, improving the stress resistance of plants. Under high P condition, the abundance of Ferrovibrio was higher in Qing Zhou Mi Tao than in Du Shi. Fe oxides considerably reduced the availability of applied P, which partially explained why the P utilization in Qing Zhou Mi Tao is inefficient. Further, Ascomycota was the dominant fungal phylum in the roots of all rootstocks under different levels of P addition. The fungi community of roots varied in different rootstocks at each P level, but was similar for the same rootstock at different P levels, which indicated that genotype had a greater effect than P addition on the fungal community of peach rootstocks.
| S-EPMC8502846 | biostudies-literature
Project description:The permeability of roots to water and nutrients is controlled through a variety of mechanisms and one of the most conspicuous is the presence of the Casparian strips and suberin lamellae. Roots actively regulate the creation of these structures developmentally, along the length of the root, and in response to the environment, including drought. In the current study, we characterized the suberin composition along the length of grapevine fine roots during development and in response to water deficit, and in the same root systems we quantified changes in expression of suberin biosynthesis- and deposition-related gene families (via RNAseq) allowing the identification of drought-responsive suberin-related genes. Grapevine suberin composition did not differ between primary and lateral roots, and was similar to that of other species. Under water deficit there was a global upregulation of suberin biosynthesis which resulted in an increase of suberin specific monomers, but without changes in their relative abundances, and this upregulation took place across all the developmental stages of fine roots. These changes corresponded to the upregulation of numerous suberin biosynthesis- and export-related genes which included orthologs of the previously characterized AtMYB41 transcriptional factor. Functional validation of two grapevine MYB41 orthologs, VriMYB41 and VriMYB41-like, confirmed their ability to globally upregulate suberin biosynthesis, export, and deposition. This study provides a detailed characterization of the developmental and water deficit induced suberization of grapevine fine roots and identifies important orthologs responsible for suberin biosynthesis, export, and its regulation in grape.
| S-EPMC7680640 | biostudies-literature
Project description:Grape growers use rootstocks to provide protection against pests and pathogens and to modulate viticulture performance such as shoot growth. Our study examined two grapevine scion varieties ('Chardonnay' and 'Cabernet Sauvignon') grafted to 15 different rootstocks and determined the effect of rootstocks on eight traits important to viticulture. We assessed the vines across five years and identified both year and variety as contributing strongly to trait variation. The effect of rootstock was relatively consistent across years and varieties, explaining between 8.99% and 9.78% of the variation in growth-related traits including yield, pruning weight, berry weight and Ravaz index (yield to pruning weight ratio). Increases in yield due to rootstock were generally the result of increases in berry weight, likely due to increased water uptake by vines grafted to a particular rootstock. We demonstrated a greater than 50% increase in yield, pruning weight, or Ravaz index by choosing the optimal rootstock, indicating that rootstock choice is crucial for grape growers looking to improve vine performance.
| S-EPMC8156960 | biostudies-literature
Project description:The endophytic microbiome of plants is believed to have a significant impact on its physiology and disease resistance, however, the role of host genotype in determining the composition of the endophytic microbiome of apple root systems remains an open question that has important implications for defining breeding objectives. In the current study, the bacterial and fungal microbiota associated with four different apple rootstocks planted in April, 2018 in the same soil environment and harvested in May, 2019 were evaluated to determine the role of genotype on the composition of both the bacterial and fungal communities. Results demonstrated a clear impact of genotype and root size on microbial composition and diversity. The fungal community was more affected by plant genotype whereas the bacterial community was shaped by root size. Fungal and bacterial abundance was equal between different-sized roots however, significantly higher microbial counts were detected in rhizosphere samples compared to root endosphere samples. This study provides information that can be used to develop a comprehensive and readily applicable understanding of the impact of genotype and environmental factors on the establishment of plant microbiome, as well as its potential function and impact on host physiology.
| S-EPMC8769040 | biostudies-literature
Project description:Plant roots support complex microbial communities that can influence nutrition, plant growth, and health. In grapevine, little is known about the impact of abiotic stresses on the belowground microbiome. In this study, we examined the drought-induced shifts in fungal composition in the root endosphere, the rhizosphere and bulk soil by internal transcribed spacer (ITS) high-throughput amplicon sequencing (HTAS). We imposed three irrigation regimes (100%, 50%, and 25% of the field capacity) to one-year old grapevine rootstock plants cv. SO4 when plants had developed 2-3 roots. Root endosphere, rhizosphere, and bulk soil samples were collected 6- and 12-months post-plantation. Drought significantly modified the overall fungal composition of all three compartments, with the root endosphere compartment showing the greatest divergence from well-watered control (100%). The overall response of the fungal microbiota associated with black-foot disease (Dactylonectria and "Cylindrocarpon" genera) and the potential biocontrol agent Trichoderma to drought stress was consistent across compartments, namely that their relative abundances were significantly higher at 50-100% than at 25% irrigation regime. We identified a significant enrichment in several fungal genera such as the arbuscular mycorrhizal fungus Funneliformis during drought at 25% watering regime within the roots. Our results reveal that drought stress, in addition to its well-characterized effects on plant physiology, also results in the restructuring of grapevine root microbial communities, and suggest the possibility that members of the altered grapevine microbiota might contribute to plant survival under extreme environmental conditions.
| S-EPMC8468433 | biostudies-literature
Project description:In the Mediterranean-type environment of Australia and other parts of the world, end-of- season or terminal drought is the most significant abiotic stress affecting wheat grain yields. This study examined the response of two wheat cultivars with contrasting root system size to terminal drought and the effect of terminal drought on grain yield and yield components. The cultivars were grown in 1.0 m deep PVC columns filled with soil in a glasshouse under well-watered conditions until the onset of ear emergence (Z51) when well-watered and terminal drought treatments were imposed. Terminal drought reduced stomatal conductance, leaf photosynthesis, and transpiration rates faster in Bahatans-87 (larger root system size) than Tincurrin (smaller root system size). Terminal drought reduced grain yield in both cultivars, more so in Bahatans-87 (80%) with the large root system than Tincurrin (67%) with the small root system, which was mainly due to a reduction in grain number and grain size in Bahatans-87 and grain size in Tincurrin. In the terminal drought treatment, Bahatans-87 had 59% lower water use efficiency than Tincurrin, as Bahatans-87 used 39% more water and reduced grain yield more than Tincurrin. The lesser reduction in grain yield in Tincurrin was associated with slower water extraction by the small root system and slower decline in stomatal conductance, leaf photosynthesis, and transpiration rates, but more importantly to faster phenological development, which enabled grain filling to be completed before the severe effects of water stress.
| S-EPMC7466772 | biostudies-literature