Dataset Information

Bacterial Diversity and Community Structure of a Municipal Solid Waste Landfill: A Source of Lignocellulolytic Potential.

ABSTRACT: Omics have given rise to research on sparsely studied microbial communities such as the landfill, lignocellulolytic microorganisms and enzymes. The bacterial diversity of Municipal Solid Waste sediments was determined using the illumina MiSeq system after DNA extraction and Polymerase chain reactions. Data analysis was used to determine the community's richness, diversity, and correlation with environmental factors. Physicochemical studies revealed sites with mesophilic and thermophilic temperature ranges and a mixture of acidic and alkaline pH values. Temperature and moisture content showed the highest correlation with the bacteria community. The bacterial analysis of the community DNA revealed 357,030 effective sequences and 1891 operational taxonomic units (OTUs) assigned. Forty phyla were found, with the dominant phyla Proteobacteria, Firmicutes, Actinobacteria, and Bacteroidota, while Aerococcus, Stenotrophomonas, and Sporosarcina were the dominant species. PICRUSt provided insight on community's metabolic function, which was narrowed down to search for lignocellulolytic enzymes' function. Cellulase, xylanase, esterase, and peroxidase were gene functions inferred from the data. This article reports on the first phylogenetic analysis of the Pulau Burung landfill bacterial community. These results will help to improve the understanding of organisms dominant in the landfill and the corresponding enzymes that contribute to lignocellulose breakdown.

SUBMITTER: Chukwuma OB

PROVIDER: S-EPMC8228822 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Project description:Groundwater pollution resulting from leachate leakage at landfill sites has garnered significant attention. Investigating the migration of pollutants from these landfills to adjacent groundwater is crucial for understanding the diffusion patterns and extent of contamination. It is imperative to develop cost-effective yet highly efficient tracer techniques to aid landfill operators in monitoring groundwater contamination stemming from their operations. The primary objective of this research was to compare the roles of conservative tracers sodium (Na+) and chloride (Cl-), and conventional pollutants permanganate oxidation (CODMn), ammonium nitrogen (NH4 +-N), lead (Pb), and zinc (Zn) in assessing pollution levels from municipal solid waste landfills to groundwater. For this purpose, a typical municipal solid landfill was selected to investigate the origin of Cl-, groundwater quality, and spatiotemporal variations of multiple contaminations. Geochemistry analyses revealed that Na-Cl and Ca-HCO3 were the dominant groundwater type in this study and landfill was the primary source of Cl- in groundwater, with an average contribution of 78 %. Groundwater in proximity to the landfill (5#, 2#, 22#, 23#) exhibited elevated concentrations of Na+ (15.6-914.0 mg/L), Cl- (8.9-1352.0 mg/L), CODMn (0.54-95.9 mg/L), and NH4 +-N (0.33-49.0 mg/L), yet demonstrated reduced levels of Pb (0.2-391.0 μg/L) and Zn (2.0-112.8 μg/L). In contrast, groundwater located at a considerable distance from the landfill (13#, 18#, 15#, 26#) displayed the inverse trend, with relatively low concentration of Na+ (3.2-8.5 mg/L), Cl- (0.1-0.7 mg/L), CODMn (0.28-4.78 mg/L), and NH4 +-N (0.03-0.52 mg/L), but increased levels of Pb (1.2-483.0 μg/L) and Zn (1.6-357.0 μg/L). The primary determinant of groundwater quality near the landfill was NH4 +-N, with the highest pollution index (Pi) of 492.85, whereas Pb was the predominant factor affecting water quality in areas distant from the landfill, with the highest pollution index (Pi) of 10.9. While no discernible seasonal variation was detected for all pollutants, spatial variation can be observed that pollution levels decreased progressively with increasing distance from the landfill, a trend particularly corroborated by the conservative Cl- and Na+ measurements. This research suggests that conservative ions, such as Cl- and Na+, exhibit superior efficacy in tracing the pollution range from municipal solid landfills to groundwater. Therefore, monitoring these conservative ions in groundwater can yield a more precise understanding of the extent of groundwater contamination originated from landfills.

Project description:Municipal solid waste incineration (MSWI) ash is often managed through co-disposal with unburned wastes in landfills, a practice previously reported to result in enhanced leaching of pollutants (e.g., heavy metals) in landfill leachate. The objective of this study was to evaluate the effect of co-disposed unburned wastes on per- and polyfluoroalkyl substances (PFAS) in MSWI ash landfill leachate. Leachate was collected from four landfills containing MSWI ash, either as a sole waste stream or co-disposed of with sewage sludge and MSW screenings. Samples of ash and unburned materials were collected and assessed separately for leachable PFAS in the laboratory. All samples were analyzed for 26 PFAS. Results showed that greater ash content was associated with lower leachate PFAS concentrations. The pure ash monofill exhibited the lowest PFAS in landfill leachate (290 ng L-1) while the landfill contained a large amount of unburned waste had the highest PFAS (11,000 ng L-1). For laboratory leaching tests, average ∑26PFAS concentration in lab ash leachate (310 ng L-1) was 10 and 24 times lower than observed in lab sewage sludge leachate (3,200 ng L-1) and lab MSW screenings leachate (7,500 ng L-1), respectively. Leachate from the ash-only landfill had ∑26PFAS concentration similar to what was measured in the ash itself. On the contrary, ∑26PFAS concentration in co-disposal landfill leachates were similar to those in PFAS-rich unburned waste itself, regardless of the percentages of landfilled unburned wastes. We hypothesize that leachate generated in co-disposal scenarios preferentially flows through PFAS-rich unburned materials and that biotransformation of precursors enhanced by unburned waste degradation further contributes to higher concentrations of terminal PFAS in ash co-disposal sites. Landfill operators should expect PFAS in leachates to be higher when PFAS-rich unburned wastes are disposed of alongside MSWI ash, even if the unburned fraction is small.

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Bacterial Diversity and Community Structure of a Municipal Solid Waste Landfill: A Source of Lignocellulolytic Potential.

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