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Cytogenomics of Deschampsia P. Beauv. (Poaceae) Species Based on Sequence Analyses and FISH Mapping of CON/COM Satellite DNA Families.

ABSTRACT: The genus Deschampsia P. Beauv. (Poaceae) involves a group of widespread polymorphic species, and many of them are highly tolerant to stressful environmental conditions. Genome diversity and chromosomal phylogeny within the genus are still insufficiently studied. Satellite DNAs, including CON/COM families, are the main components of the plant repeatome, which contribute to chromosome organization. For the first time, using PCR-based (Polymerase Chain Reaction) techniques and sequential BLAST (Basic Local Alignment Search Tool) and MSA (Multiple Sequence Alignment) analyses, we identified and classified CON/COM repeats in genomes of eleven Deschampsia accessions and three accessions from related genera. High homology of CON/COM sequences were revealed in the studied species though differences in single-nucleotide alteration profiles detected in homologous CON/COM regions indicated that they tended to diverge independently. The performed chromosome mapping of 45S rDNA, 5S rDNA, and CON/COM repeats in six Deschampsia species demonstrated interspecific variability in localization of these cytogenetic markers and facilitated the identification of different chromosomal rearrangements. Based on the obtained data, the studied Deschampsia species were distinguished into karyological groups, and MSA-based schematic trees were built, which could clarify the relationships within the genus. Our findings can be useful for further genetic and phylogenetic studies.

SUBMITTER: Amosova AV

PROVIDER: S-EPMC8229069 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Project description:Background and Aims: The role and significance of phytoliths in taxonomic diagnosis of grass species has been well documented with a focus on the types found in foliar epidermis and the synflorescence. The present paper is an attempt to broaden the scope of phytoliths in species diagnosis of grasses by developing phytolith signatures of some species of the foxtail genus Setaria P. Beauv. through in situ location and physico-chemical analysis of various phytolith morphotypes in different parts of the plant body. Methods: Clearing solution and dry ashing extraction methods were employed for in situ location and isolation of phytolith morphotypes respectively. Ultrastructural details were worked out by Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy. Morphometric and frequency data of phytolith morphotypes were also recorded. Biochemical architecture of various phytolith types was worked out through SEM-EDX, XRD, and FTIR analysis. Data were analyzed through Principal Component Analysis and Cluster Analysis. Key Results:In situ location of phytoliths revealed species specific epidermal patterns. The presence of cystoliths (calcium oxalate crystals) in the costal regions of adaxial leaf surface of S. verticillata (L.) P. Beauv. is the first report for the genus Setaria. Our results revealed marked variations in epidermal ornamentation and undulation patterns with a novel "Λ" (Lamda) type of undulated ornamentation reported in S. verticillata. Dry ashing method revealed species specific clusters of phytolith morphotypes. Conclusions: The study revealed that phytoliths can play a significant role in resolution of taxonomic identity of three species of Setaria. Each species was marked out by a unique assemblage of phytolith morphotypes from various parts of the plant body. Apart from in situ location and epidermal patterning, diagnostic shapes, frequency distribution, size dimensions, and biochemical architecture emerged as complementary traits that help in developing robust phytolith signatures for plant species.

Dataset Information

Cytogenomics of Deschampsia P. Beauv. (Poaceae) Species Based on Sequence Analyses and FISH Mapping of CON/COM Satellite DNA Families.

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