Project description:Introduction: The secondary infection of F. gigantica 4 weeks after primary infection could not induce a robust adaptive immune response in buffalo. As excretory and secretory products (ESP) show intense immune regulation and plays critical role in primary infection process, while research on ESP in secondary infection is lacking. Does the components of ESP in secondary infection was different from that of primary infection? Does fluke secrete and excrete new component to immunoregulation during secondary infection? Here, the component of F. gigantica ESP in buffalo primary and secondary infections was identified and compared, which will drive the exploration in repeat infection in basis matter, and also conducive to drug targets and vaccine candidate screening. Methods: Buffaloes were assigned to the group A (n = 3, non-infection), group B (n = 3, primary infection) and group C (n = 3, secondary infection). The primary infection group was orally administrated 250 metacercariae at 4 week post-infection (wpi), while the secondary infection group was orally administrated 250 metacercariae at 0 and 4 wpi. Then Sera were collected at 0, 10, and 16 wpi for group A, 0 (pre-infection), 1, 3, 6, 8, 10, 13, and 16 wpi for group B, and 0, 1, 3, 4, 5, 7, 10, 12, 14, 17 and 20 wpi for group C. FgESP components interacting with sera from three groups were pulled down by co-immunoprecipitated (co-IP) and identified using LC–MS/MS. Interacting FgESP components in group C were subjected to Kyoto Encyclopedia of Genes and Genomes (KEGG) metabolic pathway and gene ontology (GO) functional annotation to infer their potential functions, and also been compared with that of group B. Results and discussion: In the group C, 327 proteins were identified, of which 74 were consistently identified at 7 time points (5, 7, 10, 12, 14, 17 and 20 wpi). GO analysis shows these 74 proteins mainly functioned in biological processes, while KEGG analysis showed they mainly functioned in metabolism and cellular processing. The numbers of specific interactors identified were 2 (5 wpi), 10 (7 wpi), 0 (10 wpi), 8 (12 wpi), 1 (14 wpi), 4 (17 wpi), and 4 (20 wpi). Here, the antigenic targets in FgESP in the secondary infection process were screened over a long and intensive period of time, and these components needs further exploration. The present study will deepen the understanding of molecular F. gigantica-buffalo interactions and helpful in new potential vaccine and drug target candidates identification
2023-06-01 | PXD042634 | iProX