Ontology highlight
ABSTRACT:
SUBMITTER: Lee HJ
PROVIDER: S-EPMC8235755 | biostudies-literature | 2021 Jun
REPOSITORIES: biostudies-literature
Lee Ho Joung HJ Kim Hyun Ju HJ Lee Sang Jun SJ
International journal of molecular sciences 20210616 12
The CRISPR/Cas9 system has recently emerged as a useful gene-specific editing tool. However, this approach occasionally results in the digestion of both the DNA target and similar DNA sequences due to mismatch tolerance, which remains a significant drawback of current genome editing technologies. However, our study determined that even single-base mismatches between the target DNA and 5'-truncated sgRNAs inhibited target recognition. These results suggest that a 5'-truncated sgRNA/Cas9 complex c ...[more]