Quantification of matrix metalloproteinases MMP-8 and MMP-9 in gingival overgrowth.

ABSTRACT:

Background

Matrix metalloproteinases (MMPs) are proteolytic enzymes involved in extracellular matrix remodeling of all body tissues, including oral tissues such as gingival tissue. Expression levels of MMPs are widely studied as important biomarkers for explaining the biochemical mechanisms and evolution of many oral diseases.

Objective

Demonstrate the sensitivity, reproducibility, repeatability, and robustness of the dot blot assay for the relative quantification of MMP-8 and MMP-9 expression levels in patients with GO associated with orthodontic treatment.

Methods

A validated dot blot assay was used to compare the relative expression levels of MMP-8 and MMP-9 in gingival samples. Methodological variability, reproducibility, sensitivity and robustness were determined

SUBMITTER: Orozco-Paez J 

PROVIDER: S-EPMC8236544 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature