Reduced microbial stability in the active layer is associated with carbon loss under alpine permafrost degradation.
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ABSTRACT:
SUBMITTER: Wu MH
PROVIDER: S-EPMC8237688 | biostudies-literature |
REPOSITORIES: biostudies-literature
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Project description:The response of microbial communities to the predicted rising temperatures in alpine regions might be an important part of the ability of these ecosystems to deal with climate change. Soil microbial communities might be significantly affected by elevated temperatures, which influence the functioning of soils within high-alpine ecosystems. To evaluate the potential of the permafrost microbiome to adapt to short-term moderate and extreme warming, we set up an incubation experiment with permafrost and active soil layers from northern and southern slopes of a high-alpine mountain ridge on Muot da Barba Peider in the Swiss Alps. Soils were acclimated to increasing temperatures (4-40°C) for 26 days before being exposed to a heat shock treatment of 40°C for 4 days. Alpha-diversity in all soils increased slightly under gradual warming, from 4 to 25°C, but then dropped considerably at 40°C. Similarly, heat shock induced strong changes in microbial community structures and functioning in the active layer of soils from both northern and southern slope aspects. In contrast, permafrost soils showed only minor changes in their microbial community structures and no changes in their functioning, except regarding specific respiration activity. Shifts in microbial community structures with increasing temperature were significantly more pronounced for bacteria than for fungi, regardless of the soil origin, suggesting higher resistance of high-alpine fungi to short-term warming. Firmicutes, mainly represented by Tumebacillus and Alicyclobacillaceae OTUs, increased strongly at 40°C in active layer soils, reaching almost 50% of the total abundance. In contrast, Saccharibacteria decreased significantly with increasing temperature across all soil samples. Overall, our study highlights the divergent responses of fungal and bacterial communities to increased temperature. Fungi were highly resistant to increased temperatures compared to bacteria, and permafrost communities showed surprisingly low response to rising temperature. The unique responses were related to both site aspect and soil origin indicating that distinct differences within high-alpine soils may be driven by substrate limitation and legacy effects of soil temperatures at the field site.
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Project description:As climate cooling is increasingly regarded as important natural variability of long-term global warming trends, there is a resurging interest in understanding its impact on biodiversity and ecosystem functioning. Here, we report a soil transplant experiment from lower to higher elevations in a Tibetan alpine grassland to simulate the impact of cooling on ecosystem community structure and function. Three years of cooling resulted in reduced plant productivity and microbial functional potential (for example, carbon respiration and nutrient cycling). Microbial genetic markers associated with chemically recalcitrant carbon decomposition remained unchanged despite a decrease in genes associated with chemically labile carbon decomposition. As a consequence, cooling-associated changes correlated with a decrease in soil organic carbon (SOC). Extrapolation of these results suggests that for every 1?°C decrease in annual average air temperature, 0.1?Pg (0.3%) of SOC would be lost from the Tibetan plateau. These results demonstrate that microbial feedbacks to cooling have the potential to differentially impact chemically labile and recalcitrant carbon turnover, which could lead to strong, adverse consequences on soil C storage. Our findings are alarming, considering the frequency of short-term cooling and its scale to disrupt ecosystems and biogeochemical cycling.
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Project description:Permafrost represents a reservoir for the biodiscovery of cold-adapted proteins which are advantageous in industrial and medical settings. Comparisons between different thermo-adapted proteins can give important information for cold-adaptation bioengineering. We collected permafrost active layer samples from 34 points along a proglacial transect in southwest Greenland. We obtained a deep read coverage assembly (>164x) from nanopore and Illumina sequences for the purposes of i) analysing metagenomic and metatranscriptomic trends of the microbial community of this area, and ii) creating the Cold-Adapted Predicted Protein (CAPP) database. The community showed a similar taxonomic composition in all samples along the transect, with a solid permafrost-shaped community, rather than microbial trends typical of proglacial systems. We retrieved 69 high- and medium-quality metagenome-assembled clusters, 213 complete biosynthetic gene clusters and more than three million predicted proteins. The latter constitute the CAPP database that can provide cold-adapted protein sequence information for protein- and taxon-focused amino acid sequence modifications for the future bioengineering of cold-adapted enzymes. As an example, we focused on the enzyme polyphenol oxidase, and demonstrated how sequence variation information could inform its protein engineering.
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Project description:Arctic ecosystems are characterized by a broad range of plant functional types that are highly heterogeneous at small (~1-2 m) spatial scales. Climatic changes can impact vegetation distribution directly, and also indirectly via impacts on disturbance regimes. Consequent changes in vegetation structure and function have implications for surface energy dynamics that may alter permafrost thermal dynamics, and are therefore of interest in the context of permafrost related climate feedbacks. In this study we examine small-scale heterogeneity in soil thermal properties and ecosystem carbon and water fluxes associated with varying understory vegetation in open-canopy larch forests in northeastern Siberia. We found that lichen mats comprise 16% of understory vegetation cover on average in open canopy larch forests, and lichen abundance was inversely related to canopy cover. Relative to adjacent areas dominated by shrubs and moss, lichen mats had 2-3 times deeper permafrost thaw depths and surface soils warmer by 1-2°C in summer and less than 1°C in autumn. Despite deeper thaw depths, ecosystem respiration did not differ across vegetation types, indicating that autotrophic respiration likely dominates areas with shrubs and moss. Summertime net ecosystem exchange of CO2 was negative (i.e. net uptake) in areas with high shrub cover, while positive (i.e. net loss) in lichen mats and areas with less shrub cover. Our results highlight relationships between vegetation and soil thermal dynamics in permafrost ecosystems, and underscore the necessity of considering both vegetation and permafrost dynamics in shaping carbon cycling in permafrost ecosystems.
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