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Developing and validating a rapid assessment tool for small ruminant reproduction and production in pastoralist flocks in Kajiado, Kenya.

ABSTRACT: The collection of production performance data in small ruminants pastoralists flocks is essential to evaluate their efficiency and assess how different challenges, such as diseases, droughts or changes in land use, affect their sustainability. Although different methodologies exist, capturing these data is difficult, particularly in nomadic pastoral flocks. In this study, a method for rapid assessment of flock production performance based on farmer recall was designed, implemented and tested. Through literature review and consultation with pastoralists and key informants, a questionnaire was developed to capture small ruminant flock data disaggregated by age (≤2 years old and >2 years old), species and sex. Data on flock dynamics and on reproduction parameters were collected for a period of 12 months. A survey of 130 pastoralists with medium to large flocks was then conducted in Kajiado County (Kenya). Values were calculated for 12 reproduction performance indicators and 7 additional production performance indicators. In addition, a flock efficiency indicator (FEI) is proposed that classifies mixed flocks (i.e., with sheep and goats) into low, medium or high efficiency. Results showed that in flocks with low efficiency, the median value for net fecundity rate was 0.43 lambs/ewe (range 0.08-1.00) and 0.41 kids/doe (range 0.07-0.73), and the median production rate was 6% (range [-47%] to 20%) for sheep and 11% (range [-38%] to 0.21%) for goats. In flocks with high efficiency the median net fecundity rate was 0.77 lambs/ewe (range 0.48-2.73) and 0.88 kids/doe (range 0.49-1.80), and the median production rate was 25% (range 11-47) for sheep and 28% (range 15-46) for goats. Sixty-two (47.7%) of the pastoralists surveyed reported usually buying animals into their flocks, and, consequently were considered as 'pastoralists and traders'. Their flocks had significantly lower FEI scores (median = 7.5, interquartile range [IQR] 6-9), compared with "pastoralists only" flocks (median = 8, IQR 6-10, Kruskal-Wallis rank sum test, p-value <0.001). Since this method is based on pastoralist recall, values obtained should be considered as approximations. Nonetheless, the proposed assessment tool can be used by individuals with low resources or recording capacity, and in large scale programmes to monitor pastoralist flock dynamics, set-up benchmarking programmes, estimate the impact of diseases and shocks, identify those flocks which are most vulnerable to these shocks and evaluate the effectiveness of policies and interventions on herd performance.

SUBMITTER: Ballesteros C

PROVIDER: S-EPMC8242044 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Project description:Bovine mastitis continues to be a leading cause of heavy economic losses in the dairy industry and a public health hazard globally. This cross-sectional study investigated the prevalence, etiologies of clinical and subclinical mastitis, and associated predisposing factors in Embu and Kajiado counties in Kenya. A semistructured questionnaire was administered to 154 smallholder dairy farmers to collect data on management practices, animal factors, and disease history. A total of 395 dairy cows were initially screened for subclinical mastitis using the California mastitis test (CMT), and milk samples were aseptically collected. Both CMT positive and CMT negative samples were analyzed using conventional bacteriological isolation and identification procedures. In the present study, the overall prevalence of mastitis based on CMT and clinical examination was 80% (316/395), out of which 6.8% (27/395) was clinical mastitis, while 73.1% (289/395) was subclinical mastitis. Based on culture, the overall prevalence of clinical and subclinical mastitis was 51.6% (815/1580), 74.4% (294/395), and 76.6% (118/154) at the quarter, cow, and farm level, respectively. From the 1574 milk samples analyzed by cultured, 1016 bacteria were yielded. The predominant bacteria were coagulase-negative Staphylococcus (CNS), 42.8% (435/1016), and in decreasing order, Streptococcus species, 22.2% (226/1016), Staphylococcus aureus, 15.7% (160/1016), and Pseudomonas aeruginosa, 5.1% (52/1016), and the least was Enterobacter species, 0.7% (7/1016), while 23.7% of the sample yielded no bacterial growth. Risk factor analysis revealed that milking mastitic cows last (p=0.002), using a clean udder drying towel for each cow (p=0.033) and previous history of mastitis (p=0.046) were significantly associated with presence of mastitis. The current study has shown a relatively high prevalence of subclinical mastitis with CNS as predominant bacteria. Therefore, control measures are urgently warranted. Management factors such as milking mastitic cows last, using a clean towel for udder drying for each cow, and culling mastitic cows should be considered and included in the Kenyan mastitis control programs.

Project description:BackgroundBrucellosis occurs globally with highly variable incidence in humans from very low in North America and Western Europe to high in the Middle East and Asia. There are few data in Sub-Saharan Africa. This study estimated the incidence of human brucellosis in a pastoralist community in Kenya.MethodsBetween February 2015 and January 2016, we enrolled persons living in randomly selected households in Kajiado County. Free health care was offered at three facilities in the study area. Those who met the study clinical case definition completed a standardized questionnaire on demographics, clinical history and presentation. A blood sample was collected and tested by Rose Bengal test (RBT), then later tested at the Kenya Medical Research Institute laboratory for Brucella IgG and IgM by ELISA. Those who tested positive by both RBT and ELISA (IgG or IgM antibodies) were classified as confirmed while those that only tested positive for IgG or IgM antibodies were classified as probable. Further, sera were tested by polymerase chain reaction using a TaqMan Array Card (TAC) for a panel of pathogens causing AFI including Brucella spp. Annual incidence of brucellosis was calculated as the number of confirmed cases in one year/total number in the study population.ResultsWe enrolled a cohort of 4746 persons in 804 households. Over half (52.3%) were males and the median age was 18 years (Interquartile range (IQR) 9 months- 32 years). A total of 236 patients were enrolled at three health facilities; 64% were females and the median age was 40.5 years (IQR 28-53 years). Thirty-nine (16.5%) were positive for Brucella antibodies by IgG ELISA, 5/236 (2.1%) by IgM ELISA and 4/236 (1.7%) by RBT. Ten percent (22/217) were positive by TAC. We confirmed four (1.7%) brucellosis cases giving an annual incidence of 84/100,000 persons/year (95% CI 82, 87). The incidence did not significantly vary by gender, age and location of residence.ConclusionWe report a high incidence of brucellosis in humans among members of this pastoralist community. Brucellosis was the most common cause of febrile illness in this community.

Project description:BackgroundOne root cause of the neglect of rabies is the lack of adequate diagnostic tests in the context of low income countries. A rapid, performance friendly and low cost method to detect rabies virus (RABV) in brain samples will contribute positively to surveillance and consequently to accurate data reporting, which is presently missing in the majority of rabies endemic countries.Methodology/principal findingsWe evaluated a rapid immunodiagnostic test (RIDT) in comparison with the standard fluorescent antibody test (FAT) and confirmed the detection of the viral RNA by real time reverse transcription polymerase chain reaction (RT-qPCR). Our analysis is a multicentre approach to validate the performance of the RIDT in both a field laboratory (N'Djamena, Chad) and an international reference laboratory (Institut Pasteur, Paris, France). In the field laboratory, 48 samples from dogs were tested and in the reference laboratory setting, a total of 73 samples was tested, representing a wide diversity of RABV in terms of animal species tested (13 different species), geographical origin of isolates with special emphasis on Africa, and different phylogenetic clades. Under reference laboratory conditions, specificity was 93.3% and sensitivity was 95.3% compared to the gold standard FAT test. Under field laboratory conditions, the RIDT yielded a higher reliability than the FAT test particularly on fresh and decomposed samples. Viral RNA was later extracted directly from the test filter paper and further used successfully for sequencing and genotyping.Conclusion/significanceThe RIDT shows excellent performance qualities both in regard to user friendliness and reliability of the result. In addition, the test cassettes can be used as a vehicle to ship viral RNA to reference laboratories for further laboratory confirmation of the diagnosis and for epidemiological investigations using nucleotide sequencing. The potential for satisfactory use in remote locations is therefore very high to improve the global knowledge of rabies epidemiology. However, we suggest some changes to the protocol, as well as careful further validation, before promotion and wider use.

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Developing and validating a rapid assessment tool for small ruminant reproduction and production in pastoralist flocks in Kajiado, Kenya.

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