Unknown

Dataset Information

0

Isolation of sesquiterpenoids from Matricaria chamomilla by means of solvent assisted flavor evaporation and centrifugal partition chromatography.


ABSTRACT: The (semi)volatile fraction of Matricaria chamomilla L., an annual herbal plant from the family of Asteraceae, contains high quantities of sesquiterpenes and sesquiterpenoids. A method was developed to achieve isolation and separation of these compounds, using a combination of solvent assisted flavor evaporation (SAFE) and solid support-free liquid-liquid chromatography. The biphasic liquid solvent system n-heptane/ethyl acetate/methanol/water, 5/2/5/2 v/v/v/v (Arizona S) was elaborated as a suitable solvent system for the simultaneous separation of the target compounds. The lab-scale liquid-liquid chromatography separation performed in a countercurrent chromatography (CCC) column was successfully transferred to a semi-preparative centrifugal partition chromatography (CPC) column, which enabled the isolation of artemisia ketone, artemisia alcohol, α-bisabolone oxide A, and (E)-en-yn-dicycloether. α-Bisabolol oxide A and (Z)-en-yn-dicycloether co-eluted, but were successfully separated by subsequent size-exclusion chromatography (SEC). Similarly, spathulenol and α-bisabolol oxide B were obtained as a mixture, and were separated by means of column chromatography using silica gel as stationary phase. The isolated compounds were characterized by means of nuclear magnetic resonance spectroscopy (NMR) and gas chromatography-mass spectrometry (GC-MS).

SUBMITTER: Slavik B 

PROVIDER: S-EPMC8245379 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC6161811 | biostudies-literature
| S-EPMC10051833 | biostudies-literature
| S-EPMC6628226 | biostudies-literature
| PRJNA335536 | ENA
| PRJNA1128346 | ENA
| PRJNA908924 | ENA
| S-EPMC8572592 | biostudies-literature
| S-EPMC9415121 | biostudies-literature
| S-EPMC7412496 | biostudies-literature
| S-EPMC7729440 | biostudies-literature