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Responsive Fluorophore Aggregation Provides Spectral Contrast for Fluorescence Lifetime Imaging.


ABSTRACT: Fluorophores experience altered emission lifetimes when incorporated into and liberated from macromolecules or molecular aggregates; this trend suggests the potential for a fluorescent, responsive probe capable of undergoing self-assembly and aggregation and consequently altering the lifetime of its fluorescent moiety to provide contrast between the active and inactive probes. We developed a cyanobenzothioazole-fluorescein conjugate (1), and spectroscopically examined the lifetime changes caused by its reduction-induced aggregation in vitro. A decrease in lifetime was observed for compound 1 in a buffered system activated by the biological reducing agent glutathione, thus suggesting a possible approach for designing responsive self-aggregating lifetime imaging probes.

SUBMITTER: Schleyer KA 

PROVIDER: S-EPMC8247454 | biostudies-literature |

REPOSITORIES: biostudies-literature

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