Less Unfavorable Salt Bridges on the Enzyme Surface Result in More Organic Cosolvent Resistance.
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ABSTRACT:
SUBMITTER: Cui H
PROVIDER: S-EPMC8252522 | biostudies-literature | 2021 May
REPOSITORIES: biostudies-literature
Publications
Less Unfavorable Salt Bridges on the Enzyme Surface Result in More Organic Cosolvent Resistance.
Cui Haiyang H Eltoukhy Lobna L Zhang Lingling L Markel Ulrich U Jaeger Karl-Erich KE Davari Mehdi D MD Schwaneberg Ulrich U
Angewandte Chemie (International ed. in English) 20210407 20
Biocatalysis for the synthesis of fine chemicals is highly attractive but usually requires organic (co-)solvents (OSs). However, native enzymes often have low activity and resistance in OSs and at elevated temperatures. Herein, we report a smart salt bridge design strategy for simultaneously improving OS resistance and thermostability of the model enzyme, Bacillus subtilits Lipase A (BSLA). We combined comprehensive experimental studies of 3450 BSLA variants and molecular dynamics simulations of ...[more]
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