FUT6 deficiency compromises basophil function by selectively abrogating their sialyl-Lewis x expression.

ABSTRACT: Sialyl-Lewis x (sLe^x, CD15s) is a tetra-saccharide on the surface of leukocytes required for E-selectin-mediated rolling, a prerequisite for leukocytes to migrate out of the blood vessels. Here we show using flow cytometry that sLe^x expression on basophils and mast cell progenitors depends on fucosyltransferase 6 (FUT6). Using genetic association data analysis and qPCR, the cell type-specific defect was associated with single nucleotide polymorphisms (SNPs) in the FUT6 gene region (tagged by rs17855739 and rs778798), affecting coding sequence and/or expression level of the mRNA. Heterozygous individuals with one functional FUT6 gene harbor a mixed population of sLe^x+ and sLe^x- basophils, a phenomenon caused by random monoallelic expression (RME).

SUBMITTER: Puan KJ 

PROVIDER: S-EPMC8253766 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature