Ontology highlight
ABSTRACT:
SUBMITTER: Zhang H
PROVIDER: S-EPMC8260261 | biostudies-literature | 2021 Jun
REPOSITORIES: biostudies-literature
Bio-protocol 20210620 12
Human induced pluripotent stem cells (hiPSCs) have been extensively used in the fields of developmental biology and disease modeling. CRISPR/Cas9 gene editing in iPSC lines often has a low frequency, which hampers its application in precise allele editing of disease-associated single nucleotide polymorphisms (SNPs), especially those in the noncoding parts of the genome. Here, we present a unique workflow to engineer isogenic iPSC lines by SNP editing from heterozygous to homozygous for disease r ...[more]