Dataset Information

Selective Activation of Cortical Columns Using Multichannel Magnetic Stimulation With a Bent Flat Microwire Array.

ABSTRACT:

Objective

Cortical neural prostheses that aim to restore useful vision, hearing, and tactile sensations require the ability to selectively target different cortical regions simultaneously. Electrical stimulation via intracortical electrodes has been used to create spatial patterns of cortical activation. However, their efficacy remains limited due to the inability of conventional electrodes to confine activation to specific cortical regions around each electrode. Magnetic stimulation from single bent wires can selectively activate pyramidal neurons while avoiding passing axons, thereby confining activation to small cortical regions. This paper presents a novel bent flat microwire array and demonstrates its effectiveness for selective activation of cortical columns in mouse brain slices.

Methods

A computational model was developed to compare the spatial resolution of magnetic stimulation from bent wire arrays with 280 and 530 μm tip spacings. The same array designs were fabricated for use in electrophysiological experiments, i.e., calcium imaging (GCaMP6s) of mouse brain slices.

Results

All fabricated array designs reliably produced spatially discrete cortical activations at low stimulus amplitudes, but the 280-μm-spacing produced strong interference (constructive or destructive) at high stimulus amplitudes, thereby resulting in single strong activations or two asymmetric activations. 4-channel bent wire arrays with spacing of 340 μm avoided the interference and produced clearer spatial patterns of activation than electrodes.

Conclusion

Bent wire array designs can influence the strength or the spatial resolution of multichannel magnetic stimulation.

Significance

These results suggest that bent microwire arrays can enhance the selectivity of multichannel stimulation of brain and therefore may help to develop reliable and effective cortical neural prostheses.

SUBMITTER: Lee SW

PROVIDER: S-EPMC8262115 | biostudies-literature |

REPOSITORIES: biostudies-literature

ACCESS DATA

Similar Datasets

Project description:A characteristic pattern of abnormal brain activity is abnormally strong neuronal synchronization, as found in several brain disorders, such as tinnitus, Parkinson's disease, and epilepsy. As observed in several diseases, different therapeutic interventions may induce a placebo effect that may be strong and hinder reliable clinical evaluations. Hence, to distinguish between specific, neuromodulation-induced effects and unspecific, placebo effects, it is important to mimic the therapeutic procedure as precisely as possibly, thereby providing controls that actually lack specific effects. Coordinated Reset (CR) stimulation has been developed to specifically counteract abnormally strong synchronization by desynchronization. CR is a spatio-temporally patterned multichannel stimulation which reduces the extent of coincident neuronal activity and aims at an anti-kindling, i.e., an unlearning of both synaptic connectivity and neuronal synchrony. Apart from acute desynchronizing effects, CR may cause sustained, long-lasting desynchronizing effects, as already demonstrated in pre-clinical and clinical proof of concept studies. In this computational study, we set out to computationally develop a sham stimulation protocol for multichannel desynchronizing stimulation. To this end, we compare acute effects and long-lasting effects of six different spatio-temporally patterned stimulation protocols, including three variants of CR, using a no-stimulation condition as additional control. This is to provide an inventory of different stimulation algorithms with similar fundamental stimulation parameters (e.g., mean stimulation rates) but qualitatively different acute and/or long-lasting effects. Stimulation protocols sharing basic parameters, but inducing nevertheless completely different or even no acute effects and/or after-effects, might serve as controls to validate the specific effects of particular desynchronizing protocols such as CR. In particular, based on our computational findings we propose a multichannel sham (i.e., inactive) stimulation protocol as control condition for phase 2 and phase 3 studies with desynchronizing multichannel stimulation techniques.