α-Particle-induced DNA damage tracks in peripheral blood mononuclear cells of [²²³Ra]RaCl₂-treated prostate cancer patients.

ABSTRACT:

Purpose

One therapy option for prostate cancer patients with bone metastases is the use of [²²³Ra]RaCl₂. The α-emitter ²²³Ra creates DNA damage tracks along α-particle trajectories (α-tracks) in exposed cells that can be revealed by immunofluorescent staining of γ-H2AX+53BP1 DNA double-strand break markers. We investigated the time- and absorbed dose-dependency of the number of α-tracks in peripheral blood mononuclear cells (PBMCs) of patients undergoing their first therapy with [²²³Ra]RaCl₂.

Methods

Multiple blood samples from nine prostate cancer patients were collected before and after administration of [²²³Ra]RaCl₂, up to 4 weeks after treatment. γ-H2AX- and 53BP1-positive α-tracks were microsco

SUBMITTER: Schumann S 

PROVIDER: S-EPMC8263441 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature