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Barcoding pest species in a biodiversity hot-spot: the South African polyphagous broad-nosed weevils (Coleoptera, Curculionidae, Entiminae).

ABSTRACT: Polyphagous broad nosed weevils (Curculionidae: Entiminae) constitute a large and taxonomically challenging subfamily that contains economically significant agricultural pests worldwide. South Africa is a hot-spot for biodiversity and several species of indigenous and endemic genera of Entiminae have shifted on to cultivated plants, with some being phytosanitary pests. The sporadic pest status of many species (where the species has an occasional economic impact on the agricultural industry, but is not encountered often enough that is is readily recognisable by researchers and agricultural extension workers) and the presence of pest complexes and cryptic species represent an identification challenge to non-specialists. Furthermore, no comprehensive identification tools exist to identify immature stages that may be found in crops/soil. In this paper, a curated barcoding database with 70 COI sequences from 41 species (39 Entiminae, 2 Cyclominae) is initiated, to assist with the complexity of identification of species in this group.

SUBMITTER: Hansen S

PROVIDER: S-EPMC8263552 | biostudies-literature |

REPOSITORIES: biostudies-literature

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New genus and species of broad-nosed weevils from Baltic amber and notes on fossils of the subfamily Entiminae (Coleoptera, Curculionidae).

Project description:Arostropsis groehnigen. et sp. n. is described from Baltic amber and temporarily placed in the tribe Naupactini. It differs from all recent Naupactini genera with open corbels by very short and flattened scape, distinct lateral carina of the pronotum and elytra, and the rostrum distinctly narrower than the head capsule. The shape of head in the extinct genus is somewhat similar to that of the extant Naupactini genera with enclosed corbels (Platyomus Sahlberg, 1823 and Aptolemus Schoenherr, 1842), but differs in the slender body, open corbels, very short antennal scape and epifrons without a median sulcus (only a longitudinal depression is slightly visible). It is also similar to the Tanymecine genus Pandeleteius Schoenherr, 1834 in general appearance, but distinct by the straight anterior edge of the pronotum, lack of postocular spurs, lobes, and vibrissae, a slightly sloping elytral declivity, lateral ridges on the pronotum, subflattened antennal scape, elongate rostrum, and sparsely setose epistome. A new synonymy of the generic names Protonaupactus Zherikhin, 1971 and Sucinophyllobius Wanat & Borowiec, 1986, syn. n., is established. The Madagascan genus Corecaulus Fairmaire, 1903 is transferred from the tribe Naupactini to the Brachyderini because of its connate claws and the similarity in chaetotaxy of the epistomal area with African and Madagascar Brachyderini genera. A key to the identification of known Baltic amber genera of Entiminae is proposed. A checklist of the prepleistocene fossil Entiminae, based on V.V. Zherikhin's data, with remarks and corrections, is presented.

| S-EPMC3253632 | biostudies-literature

Austromonticola, a new genus of broad-nosed weevil (Coleoptera, Curculionidae, Entiminae) from montane areas of New Zealand.

Project description:Austromonticolagen. n. is proposed for a group of eight New Zealand alpine broad-nosed weevil species, all of which are here described: A. atriariussp. n. (type locality: Umbrella Mountains, Central Otago), A. caelibatussp. n. (type locality: Ohau Range, Mackenzie), A. furcatussp. n. (type locality: Old Man Range, Central Otago), A. inflatussp. n. (type locality: Hawkdun Range, Central Otago), A. planulatussp. n. (type locality: St Marys Range, Central Otago), A. postinventussp. n. (type locality: Kirkliston Range, South Canterbury), A. mataurasp. n. (type locality: Mt Dick, Otago Lakes) and A. rotundussp. n. (type locality: Old Man Range, Central Otago). All species occur exclusively above 1000 m elevation in the mountains of Central Otago and South Canterbury in the South Island. A phylogeny of the genus, including six outgroups, was inferred from 33 morphological characters. It resolved the genus as monophyletic, and revealed two strongly supported clades within Austromonticola. DNA sequences of four gene regions were obtained from five species. Of these, the 3' end of COI proved to be the most suitable for the identification of specimens. Females of all species have diagnostic secondary sexual structures on the elytra and ventrites. These structures are hypothesised to have evolved to assist with oviposition in and beside cushion plants or by selection for structures to mitigate the costs to females of prolonged mating.

| S-EPMC5674147 | biostudies-literature

Newly Emerging Pest in China, Rhynchaenusmaculosus (Coleoptera: Curculionidae): Morphology and Molecular Identification with DNA Barcoding.

Project description:The oak flea weevil, Rhynchaenusmaculosus Yang et Zhang 1991, is a newly emerging pest that severely damages oak (genus Quercus) in China. The first R. maculosus outbreak occurred in 2020 and caused spectacular damage to all oak forests in Jilin province, northeast China. The lack of key morphological characters complicates the identification of this native pest, especially in larva and pupa stages. This is problematic because quick and accurate species identification is crucial for early monitoring and intervention during outbreaks. Here, we provided the first detailed morphological description of R. maculosus at four life stages. Additionally, we used DNA barcodes from larva and pupa specimens collected from three remote locations for molecular identification. The average pairwise divergence of all sequences in this study was 0.51%, well below the 2% to 3% (K-2-parameter) threshold set for one species. All sample sequences matched the R. maculosus morphospecies (KX657706.1 and KX657707.1), with 99.23% to 100% (sequence identity, E value: 0.00) matching success. The tree based on barcodes placed the specimens into the Rhynchaenus group, and the phylogenetic relationship between 62 sequences (30 samples and 32 from GeneBank) had high congruence with the morphospecies taxa. The traditional DNA barcodes were successfully transformed into quick response codes with larger coding capacity for information storage. The results showed that DNA barcoding is reliable for R. maculosus identification. The integration of molecular and morphology-based methods contributes to accurate species identification of this newly emerging oak pest.

| S-EPMC8235397 | biostudies-literature

On Chinese Trachyphloeini with description of four new species (Coleoptera, Curculionidae, Entiminae).

Project description:Rhinodontodes alashanensis sp. nov., Trachyphloeosoma honza sp. nov., T. jirka sp. nov., and T. martin sp. nov. are described from China, illustrated and compared with similar species. The genus Rhinodontodes and the species Rhinodontodes subsignatus Voss, 1967 and Rhinodontus mongolicus Borovec, 2003 are recorded from China for the first time. Keys to all Chinese genera of Trachyphloeini, and to the Chinese species of Rhinodontodes and Trachyphloeosoma, are provided.

| S-EPMC7557530 | biostudies-literature

The genus Apodrosus Marshall, 1922 in Cuba (Coleoptera, Curculionidae, Entiminae, Polydrusini).

Project description:The genus Apodrosus Marshall is newly recorded for, and revised for Cuba. Nine new species are recognized as follows: Apodrosus alberti (type locality, Granma, Parque Nacional Pico Turquino), A. alternatus (type locality, Guantánamo, El Yunque), A. franklyni (type locality, Cienfuegos, Parque Nacional Pico San Juan), A. griseus (type locality, Santiago de Cuba, Siboney-Jutici Ecological Reserve), A. mensurensis (type locality, Holguin, Parque Nacional La Mensura-Piloto), A. pseudoalternatus (type locality, Matanzas, Varahicacos), A. beckeli (type locality, Guantánamo, 8 km W. Imias), A. sandersoni (type locality, Guantánamo, Loma Lafarola), and A. zayasi (type locality, Cienfuegos, Parque Nacional Pico San Juan). A key for their identification, descriptions, summaries of natural history information and data on distributions are presented. A molecular phylogeny based on 11 species of Apodrosus from Cuba, Hispaniola and Puerto Rico is reconstructed. A sister group relationship between Polydrusus and Apodrosus is recovered with a limited sampling of the former genus. The monophyly of Apodrosus is recovered with strong support. Cuban Apodrosus are not monophyletic. Five of the six sampled Cuban species form a clade, sister to an undescribed Apodrosus species from the Dominican Republic; and, Apodrosus alternatus is sister to A. quisqueyanus Girón & Franz, 2010, a species from the Dominican Republic. Biogeographic implications for Cuban species are discussed in light of the phylogeny.

| S-EPMC5523398 | biostudies-literature

Mitochondrial genome of Sitona callosus (Coleoptera: Curculionidae) and phylogenetic analysis within Entiminae.

Project description:In this study, we sequenced and annotated the nearly complete mitochondrial genome (mitogenome) of Sitona callosus (Coleoptera: Curculionidae). This mitogenome was 14,333?bp long and encoded 13 protein-coding genes, 19 transfer RNA genes (tRNAs), and two ribosomal RNA unit genes. Gene rearrangements were presented in a tRNA cluster of six tRNAs between nad3 and nad5, i.e. the ancestral order ARNSEF was changed to be RNSAEF. All tRNAs had a typical secondary cloverleaf structure, except for trnS1 which lacked the dihydrouridine arm. The Bayesian phylogenetic tree of 11 Entiminae species based on the concatenated nucleotide sequences of 13 PCGs showed that S. callosus and S. lineatus formed a clade which was at the basal position in the Entiminae phylogeny.

| S-EPMC7800592 | biostudies-literature

Systematic revision of the genus Isodacrys Sharp, 1911 (Coleoptera: Curculionidae: Entiminae: Tanymecini).

Project description:The genus Isodacrys Sharp, 1911 is revised. Twenty species of the genus are recognized ranging from south United States of America, Mexico, Guatemala and Honduras of which eight are herein described as new. These species are Isodacrys antrum Cortés-Hernández, new species (Mexico: Tamaulipas, Chiapas; Guatemala: Baja Verapaz); Isodacrys carlae Cortés-Hernández, new species (Mexico: Coahuila, Hidalgo, Nuevo León, San Luis Potosí, Tamaulipas); Isodacrys confusum Cortés-Hernández, new species (Mexico: Tamaulipas); Isodacrys fasciatum Cortés-Hernández, new species (Mexico: Coahuila, Durango, Nuevo León); Isodacrys frontalis Cortés-Hernández, new species (Mexico: Oaxaca; Guatemala: Sacatepéquez, Guatemala); Isodacrys kuchii Cortés-Hernández, new species (Mexico: Puebla); Isodacrys obrienorum Cortés-Hernández, new species (Guatemala: Totonicapán, Jalapa, San Marcos); and Isodacrys okuiltontli Cortés Hernández, new species (Mexico: Oaxaca). Insights into the monophyly of Isodacrys and its phylogenetic relationships with other Tanymecini based on adult morphology are given by implementing a phylogenetic analysis of 43 terminals (21 ingroup, 22 outgroup) coded for 72 adult morphological characters. Characters were discussed and highlighted for the inclusion in the phylogenetic analysis. Final analysis yielded two most-parsimonious cladograms of 242 steps, which support the monophyly of Isodacrys. Isodillex Cortés-Hernández, new genus is here described to accommodate Isodillex minutum (Sharp, 1911), new combination and Isodillex plumosum Cortés-Hernández, new species (Mexico: Zacatecas). Isodillex was recovered as sister group of Isodacrys. Key to separate Isodacrys species, occurrence map and habitus photographs are also provided.

| S-EPMC7602693 | biostudies-literature

Identifying the Genetic Distance Threshold for Entiminae (Coleoptera: Curculionidae) Species Delimitation via COI Barcodes.

Project description:The subfamily Entiminae is the largest group in the family Curculionidae, and it has long represented a challenge in traditional and molecular classification. Here, we analyzed intra- and interspecific genetic distances of 621 public COI barcode sequences (658bp) from 39 genera and 110 species of Entiminae, to determine parameters most congruent in retaining established species. We found that the mean intraspecific genetic distance (3.07%) was much smaller than the mean interspecific one (21.96%), but there is a wide range of overlap between intra- and interspecific genetic distances (0.77−18.01%), indicating that there is no consistent, universal barcoding gap. Specifically, DNA barcoding gap analysis for morphospecies revealed that 102 of 110 morphospecies had barcoding gaps, and 9.18% was the optimum threshold of genetic distances for 97 species delimitation. We further confirmed this threshold with barcodes from 27 morphologically identified specimens (including 21 newly reported barcodes) sequenced from five genera and seven species. We also identified thresholds to delimit congeneric species within 14 selected genera (species > 2), which varied from 7.42% (Trichalophus) to 13.48% (Barypeithes). We herein present optimal parameters for species identification in the Entiminae. Our study suggests that despite no universal genetic distance threshold value in subfamily Entiminae, 9.18% is optimal for most species. We recommend a wider sampling of geographic populations to better account for intraspecific distance variation, and that genetic distance thresholds for species delimitation should be refined at the genus level.

| S-EPMC8953793 | biostudies-literature

Review of the species of Leptomias Faust from Sichuan, China (Coleoptera, Curculionidae, Entiminae).

Project description:An account is given of the twelve species of Leptomias Faust, 1886 occurring in the Sichuan Province of China, including the description of a new species, Leptomias verticalis Ren, Zhang & Song, sp. n. from Jiulong County, Southwest Sichuan. New locality data and remarks for the other eleven species, a key to and distribution map of all twelve Sichuan species are provided. Leptomias chenae Alonso-Zarazaga & Ren is transferred to Geotragus Schoenherr, 1845, where its valid name is G. granulatus (Chao, 1980), comb. n. in application of Art. 59.4. Structural details of Leptomias verticalis and Geotragus granulatus are illustrated.

| S-EPMC5523364 | biostudies-literature

Genetic characterization of 2 Ceutorhynchus (Coleoptera: Curculionidae) weevils with mitogenomes and insights into the phylogeny and evolution of related weevils.

Project description:The rape stem weevil (Ceutorhynchus asper Roel.) and its close relatives primarily breed on cruciferous plants and cause severe damage to rapeseed production. However, their genetic and molecular information is still scarce. Here, we generated mitogenomes for both C. asper and Ceutorhynchus albosuturalis. The lengths of the 2 mitochondrial genomes are 14,207 bp (C. asper) and 15,373 bp (C. albosuturalis), and both weevils exhibit identical numbers of protein-coding genes with the absence of trnI. A + T contents for both mitogenomes are high (80% and 79.9%, respectively). Haplotype and genetic distance analyses showed that the genetic differentiation of C. asper populations in northwestern China is low. Based on 5 datasets from mitogenomes, phylogenetic analyses with maximum-likelihood and Bayesian methods show that both species (C. asper and C. albosuturalis) fall in the CCCMS clade (Curculioninae, Conoderinae, Cossoninae, Molytinae, and Scolytinae) of Curculionidae and belong to clades H and I of the genus Ceutorhynchus, respectively. Larvae of the clade H weevils mainly are borers in petioles or stems of cruciferous plants, while larvae of the clade I weevils mainly inhabit the fruits of the same plants, suggesting that ecological niche specialization can play a critical role in the diversification of Ceutorhynchus species. This study generates baseline molecular and genetic information for future research of Ceutorhynchus-related taxa and provides insights into the phylogeny and evolution of Curculionidae.

| S-EPMC10972553 | biostudies-literature

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