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Lack of evidence for infectious SARS-CoV-2 in feces and sewage.

ABSTRACT: The SARS-CoV-2 can be excreted in feces and can reach sewage systems. Determining the presence of infective viral particles in feces and sewage is necessary to take adequate control measures and to elucidate new routes of transmission. Here, we have developed a sample concentration methodology that allows us to maintain viral infectivity. Feces of COVID-19 patients and wastewater samples have been analyzed both by molecular methods and cell culture. Our results show no evidence of infective viral particles, suggesting that fecal-oral transmission is not a primary route. However, larger-scale efforts are needed, especially with the emergence of new viral variants.

SUBMITTER: Albert S

PROVIDER: S-EPMC8266481 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature

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Lack of evidence for infectious SARS-CoV-2 in feces and sewage.

Albert Sandra S Ruíz Alba A Pemán Javier J Salavert Miguel M Domingo-Calap Pilar P

European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology 20210709 12

The SARS-CoV-2 can be excreted in feces and can reach sewage systems. Determining the presence of infective viral particles in feces and sewage is necessary to take adequate control measures and to elucidate new routes of transmission. Here, we have developed a sample concentration methodology that allows us to maintain viral infectivity. Feces of COVID-19 patients and wastewater samples have been analyzed both by molecular methods and cell culture. Our results show no evidence of infective vira ...[more]

PMID: 34240259

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