NORAD-induced Pumilio phase separation is required for genome stability.

ABSTRACT: Liquid-liquid phase separation is a major mechanism of subcellular compartmentalization^1,2. Although the segregation of RNA into phase-separated condensates broadly affects RNA metabolism^3,4, whether and how specific RNAs use phase separation to regulate interacting factors such as RNA-binding proteins (RBPs), and the phenotypic consequences of such regulatory interactions, are poorly understood. Here we show that RNA-driven phase separation is a key mechanism through which a long noncoding RNA (lncRNA) controls the activity of RBPs and maintains genomic stability in mammalian cells. The lncRNA NORAD prevents aberrant mitosis by inhibiting Pumilio (PUM) proteins^5-8. We show that NORAD can out-compete thousands of other PUM-binding transcripts to inhibit PUM

SUBMITTER: Elguindy MM 

PROVIDER: S-EPMC8266761 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature