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Microalgal feedstock for the production of omega-3 fatty acid ethyl esters and ɛ-polylysine.

ABSTRACT: Microalgal omega-3 fatty acids are considered as an efficient alternative for fish-based omega-3 fatty acids. Ethyl esters derived from omega-3 fatty acids are being considered as the drug for hypertriglyceridemia. In this study, omega-3 fatty acids rich Chlorella sp. was utilized for the transesterification for the ethyl ester production using a potassium carbonate alkaline catalyst. At the optimized conditions of transesterification, 86.2% ethyl ester yield was achieved with solvent to algae ratio (20 mL/g), water addition (45 %), catalyst (4 %), temperature (75°C), and reaction time (60 min). Additionally, the acid-hydrolysed spent biomass was used for the production of ɛ-polylysine by fermentation using Streptomyces sp. as fermentative organism. The maximum yield of 1.78 g/L was achieved after 90 h fermentation. This study established a biorefinery approach where two highly valuable compounds could be produced from the Chlorella sp. by transesterification followed by fermentation.

SUBMITTER: Sivaramakrishnan R

PROVIDER: S-EPMC8267437 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature

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Microalgal feedstock for the production of omega-3 fatty acid ethyl esters and ɛ-polylysine.

Sivaramakrishnan Ramachandran R Ramadoss Govindarajan G Suresh Subramaniyam S Poornima Sivamani S Pugazhendhi Arivalagan A Incharoensakdi Aran A

Biotechnology reports (Amsterdam, Netherlands) 20210629

Microalgal omega-3 fatty acids are considered as an efficient alternative for fish-based omega-3 fatty acids. Ethyl esters derived from omega-3 fatty acids are being considered as the drug for hypertriglyceridemia. In this study, omega-3 fatty acids rich <i>Chlorella</i> sp. was utilized for the transesterification for the ethyl ester production using a potassium carbonate alkaline catalyst. At the optimized conditions of transesterification, 86.2% ethyl ester yield was achieved with solvent to ...[more]

PMID: 34277365

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Project description:BackgroundOmega-3 fatty acids from fish oil have been associated with beneficial cardiovascular effects, but their role in modifying cardiac structures and tissue characteristics in patients who have had an acute myocardial infarction while receiving current guideline-based therapy remains unknown.MethodsIn a multicenter, double-blind, placebo-controlled trial, participants presenting with an acute myocardial infarction were randomly assigned 1:1 to 6 months of high-dose omega-3 fatty acids (n=180) or placebo (n=178). Cardiac magnetic resonance imaging was used to assess cardiac structure and tissue characteristics at baseline and after study therapy. The primary study endpoint was change in left ventricular systolic volume index. Secondary endpoints included change in noninfarct myocardial fibrosis, left ventricular ejection fraction, and infarct size.ResultsBy intention-to-treat analysis, patients randomly assigned to omega-3 fatty acids experienced a significant reduction of left ventricular systolic volume index (-5.8%, P=0.017), and noninfarct myocardial fibrosis (-5.6%, P=0.026) in comparison with placebo. Per-protocol analysis revealed that those patients who achieved the highest quartile increase in red blood cell omega-3 index experienced a 13% reduction in left ventricular systolic volume index in comparison with the lowest quartile. In addition, patients in the omega-3 fatty acid arm underwent significant reductions in serum biomarkers of systemic and vascular inflammation and myocardial fibrosis. There were no adverse events associated with high-dose omega-3 fatty acid therapy.ConclusionsTreatment of patients with acute myocardial infarction with high-dose omega-3 fatty acids was associated with reduction of adverse left ventricular remodeling, noninfarct myocardial fibrosis, and serum biomarkers of systemic inflammation beyond current guideline-based standard of care.Clinical trial registrationURL: http://www.clinicaltrials.gov. Unique identifier: NCT00729430.

Project description:IntroductionPatients with diabetes and prediabetes are at increased risk of dyslipidemia and cardiovascular disease. To reduce this risk, statins and additional therapies may be considered. Omega-3 fatty acids offer an option to reduce triglycerides (TG) and potentially improve other lipid parameters, although products that contain docosahexaenoic acid (DHA) may increase low-density lipoprotein cholesterol (LDL-C) while eicosapentaenoic acid (EPA) does not. Prescription formulations include omega-3-acid mixtures (combination of predominantly EPA and DHA), and icosapent ethyl (high-purity prescription form of EPA ethyl ester); prescription omega-3 products are indicated as an adjunct to diet to reduce TGs in adult patients with severe hypertriglyceridemia at a dose of 4 g/day.MethodsThis was a retrospective analysis of records from a private endocrinology practice of patients who received omega-3-acid ethyl esters (OM3EE) (4 g/day) and were subsequently switched to icosapent ethyl (IPE; 4 g/day) due to the potential of OM3EE to raise LDL-C and/or cause gastrointestinal upset. Patient records were analyzed for LDL-C, TG, total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), and non-HDL-C measured before and after the switch to IPE.ResultsThe records of ten patients met the criteria for this analysis and were included. All patients had taken OM3EE for ≥1 year prior to their last lipid measurement before switching to IPE, and all had been taking IPE for >3 months at the time of their subsequent lipid measurement. Nine of the ten patients were on concomitant statin therapy throughout. Reductions in LDL-C, TC, and non-HDL-C were observed in eight patients, reductions or no changes in TG were observed in eight patients, and increases or no changes in HDL-C were observed in eight patients. No gastrointestinal adverse events were observed.ConclusionIn most patients with prediabetes or diabetes who switched from OM3EE to IPE, LDL-C and other lipid parameters improved. IPE was well tolerated.

Dataset Information

Microalgal feedstock for the production of omega-3 fatty acid ethyl esters and ɛ-polylysine.

Publications

Microalgal feedstock for the production of omega-3 fatty acid ethyl esters and ɛ-polylysine.

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