ChIP-seq Experiment and Data Analysis in the Cyanobacterium Synechocystis sp. PCC 6803.
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SUBMITTER: Giner-Lamia J
PROVIDER: S-EPMC8275305 | biostudies-literature | 2018 Jun
REPOSITORIES: biostudies-literature
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ChIP-seq Experiment and Data Analysis in the Cyanobacterium <i>Synechocystis</i> sp. PCC 6803.
Giner-Lamia Joaquín J Hernández-Prieto Miguel A MA Futschik Matthias E ME
Bio-protocol 20180620 12
Nitrogen is an essential nutrient for all living organisms. In cyanobacteria, a group of oxygenic photosynthetic bacteria, nitrogen homeostasis is maintained by an intricate regulatory network around the transcription factor NtcA. Although mechanisms controlling NtcA activity appear to be well understood, the sets of genes under its control (<i>i.e.</i>, its regulon) remain poorly defined. In this protocol, we describe the procedure for chromatin immunoprecipitation using NtcA antibodies, follow ...[more]
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