Analyses of Gnai3-iresGFP reporter mice reveal unknown Gα_i3 expression sites.

ABSTRACT: Inhibitory G proteins (G_i proteins) are highly homologous but play distinct biological roles. However, their isoform-specific detection remains challenging. To facilitate the analysis of Gα_i3 expression, we generated a Gnai3- iresGFP reporter mouse line. An internal ribosomal entry site (IRES) was inserted behind the stop-codon of the Gnai3 gene to initiate simultaneous translation of the GFP cDNA together with Gα_i3. The expression of GFP was confirmed in spleen and thymus tissue by immunoblot analysis. Importantly, the GFP knock-in (ki) did not alter Gα_i3 expression levels in all organs tested including spleen and thymus compared to wild-type littermates. Flow cytometry of thymocytes, splenic and blood cell suspensions revealed significantly

SUBMITTER: Leiss V 

PROVIDER: S-EPMC8275620 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature