Unknown

Dataset Information

0

Optimizing the microscopic agglutination test (MAT) panel for the diagnosis of Leptospirosis in a low resource, hyper-endemic setting with varied microgeographic variation in reactivity.


ABSTRACT: The microscopic agglutination test (MAT) is the standard serological reference test for the diagnosis of leptospirosis, despite being a technically demanding and laborious procedure. The use of a locally optimised MAT panel is considered essential for proper performance and interpretation of results. This paper describes the procedure of selecting such an optimised panel for Sri Lanka, a country hyper-endemic for leptospirosis. MAT was performed using 24 strains on 1132 serum samples collected from patients presenting with acute undifferentiated fever. Of 24 strains, 15 were selected as the optimised panel, while only 11% of serum samples showed positivity. A geographical variation in predominantly reactive serovars was observed, whereas reactivity was low with the saprophytic strain Patoc. Testing with paired sera yielded a higher sensitivity but provided only a retrospective diagnosis. Serological tests based on ELISA with complementary molecular diagnosis using PCR are a feasible and robust alternative approach to diagnose leptospirosis in countries having a higher burden of the disease.

SUBMITTER: Jayasundara D 

PROVIDER: S-EPMC8279374 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature

altmetric image

Publications

Optimizing the microscopic agglutination test (MAT) panel for the diagnosis of Leptospirosis in a low resource, hyper-endemic setting with varied microgeographic variation in reactivity.

Jayasundara Dinesha D   Gamage Chandika C   Senavirathna Indika I   Warnasekara Janith J   Matthias Michael A MA   Vinetz Joseph M JM   Agampodi Suneth S  

PLoS neglected tropical diseases 20210701 7


The microscopic agglutination test (MAT) is the standard serological reference test for the diagnosis of leptospirosis, despite being a technically demanding and laborious procedure. The use of a locally optimised MAT panel is considered essential for proper performance and interpretation of results. This paper describes the procedure of selecting such an optimised panel for Sri Lanka, a country hyper-endemic for leptospirosis. MAT was performed using 24 strains on 1132 serum samples collected f  ...[more]

Similar Datasets

| S-EPMC8594833 | biostudies-literature
| S-EPMC4472754 | biostudies-literature
| S-EPMC9221883 | biostudies-literature
| S-EPMC7965942 | biostudies-literature
| S-EPMC4816860 | biostudies-literature
| S-EPMC9503138 | biostudies-literature
| S-EPMC10848445 | biostudies-literature
| S-EPMC7485768 | biostudies-literature
| S-EPMC9325526 | biostudies-literature
| S-EPMC8654203 | biostudies-literature