Browse
Submit Data
Databases
API
Help

Dataset Information

21 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Enhancement of seed germination and microbial disinfection on ginseng by cold plasma treatment.

ABSTRACT:

Background

This study aimed to investigate the effect of cold plasma treatment on the improvement of seed germination and surface sterilization of ginseng seeds.

Methods

Dehisced ginseng (Panax ginseng) seeds were exposed to dielectric barrier discharge (DBD) plasma operated in argon (Ar) or an argon/oxygen mixture (Ar/O₂), and the resulting germination and surface sterilization were compared with those of an untreated control group. Bacterial and fungal detection assays were performed for plasma-treated ginseng seeds after serial dilution of surface-washed suspensions. The microbial colonies (fungi and bacteria) were classified according to their phenotypical morphologies and identified by molecular analysis. Furthermore, the effect of cold plasma treatment on the in vitro antifungal activity and suppression of Cylindrocarpon destructans in 4-year-old ginseng root discs was investigated.

Results

Seeds treated with plasma in Ar or Ar/O₂ exhibited a higher germination rate (%) compared with the untreated controls. Furthermore, the plasma treatment exhibited bactericidal and fungicidal effects on the seed surface, and the latter effect was stronger than the former. In addition, plasma treatment exhibited in vitro antifungal activity against C. destructans and reduced the disease severity (%) of root rot in 4-year-old ginseng root discs. The results demonstrate the stimulatory effect of plasma treatment on seed germination, surface sterilization, and root rot disease suppression in ginseng.

Conclusion

The results of this study indicate that the cold plasma treatment can suppress the microbial community on the seed surface root rot in ginseng.

SUBMITTER: Lee Y

PROVIDER: S-EPMC8282493 | biostudies-literature |

REPOSITORIES: biostudies-literature

ACCESS DATA

Json Xml

Similar Datasets

Cold plasma treatment for cotton seed germination improvement.

Project description:Adverse environmental conditions at planting, such as cold temperature or water limitation, can lead to a reduced level of seed germination and plant establishment for cotton. Cold atmospheric-pressure plasma (CAP) treatment of cotton seeds prior to planting may help alleviate this problem. CAP is ionised gas that has a range of biological activities due to the formation of a mix of reactive oxygen and nitrogen species (RONS), excited molecules, charged particles and UV photons. Our results show that a 27?minutes CAP treatment using air can significantly increase water absorption of the seed, and improve warm germination, metabolic chill test germination and chilling tolerance in cotton. We also observe that the beneficial effect of CAP treatment is long-lasting and stable as improved germination activity is still seen when treatment occurs 4 months before germination testing, suggesting that future large-scale industrial seed plasma treatments may still be effectively applied well (months) before the seed planting. We conclude that CAP treatment is a promising new tool for use in the cotton industry that has the potential to significantly improve plant establishment in a wider range of environmental conditions.

| S-EPMC6158256 | biostudies-literature

Cold plasma treatment enhances oilseed rape seed germination under drought stress.

Project description:Effects of cold plasma treatment on seed germination, seedling growth, antioxidant enzymes, lipid peroxidation levels and osmotic-adjustment products of oilseed rape under drought stress were investigated in a drought-sensitive (Zhongshuang 7) and drought-tolerant cultivar (Zhongshuang 11). Results showed that, under drought stress, cold plasma treatment significantly improved the germination rate by 6.25% in Zhongshuang 7, and 4.44% in Zhongshuang 11. Seedling growth characteristics, including shoot and root dry weights, shoot and root lengths, and lateral root number, significantly increased after cold plasma treatment. The apparent contact angle was reduced by 30.38% in Zhongshuang 7 and 16.91% in Zhongshuang 11. Cold plasma treatment markedly raised superoxide dismutase and catalase activities by 17.71% and 16.52% in Zhongshuang 7, and by 13.00% and 13.21% in Zhongshuang 11. Moreover, cold plasma treatment significantly increased the soluble sugar and protein contents, but reduced the malondialdehyde content in seedlings. Our results suggested that cold plasma treatment improved oilseed rape drought tolerance by improving antioxidant enzyme activities, increasing osmotic-adjustment products, and reducing lipid peroxidation, especially in the drought-sensitive cultivar (Zhongshuang 7). Thus, cold plasma treatment can be used in an ameliorative way to improve germination and protect oilseed rape seedlings against damage caused by drought stress.

| S-EPMC4533018 | biostudies-other

Effects of cold plasma treatment on seed germination and seedling growth of soybean.

Project description:Effects of cold plasma treatment on soybean (Glycine max L. Merr cv. Zhongdou 40) seed germination and seedling growth were studied. Seeds were pre-treated with 0, 60, 80, 100 and 120 W of cold plasma for 15 s. Results showed that plasma treatments had positive effects on seed germination and seedling growth, and treatment of 80 W had the highest stimulatory effect. Germination and vigor indices significantly increased by 14.66% and 63.33%, respectively. Seed's water uptake improved by 14.03%, and apparent contact angle decreased by 26.19%. Characteristics of seedling growth, including shoot length, shoot dry weight, root length and root dry weight, significantly increased by 13.77%, 21.95%, 21.42% and 27.51%, respectively, compared with control. The seed reserve utilization, including weight of the mobilized seed reserve, seed reserve depletion percentage and seed reserve utilization efficiency significantly improved by cold plasma treatment. In addition, soluble sugar and protein contents were 16.51% and 25.08% higher than those of the control. Compared to a 21.95% increase in shoot weight, the root weight increased by 27.51% after treatment, indicating that plasma treatment had a greater stimulatory effect on plant roots. These results indicated that cold plasma treatment might promote the growth even yield of soybean.

| S-EPMC4118186 | biostudies-other

Evaluation of the Impact of Cold Atmospheric Pressure Plasma on Soybean Seed Germination.

Project description:The present study aims to define the effects of Cold Atmospheric Pressure Plasma (CAPP) exposure on seed germination of an agriculturally important crop, soybean. Seed treatment with lower doses of CAPP generated in ambient air and oxygen significantly increased the activity of succinate dehydrogenase (Krebs cycle enzyme), proving the switching of the germinating seed metabolism from anoxygenic to oxygenic. In these treatments, a positive effect on seed germination was documented (the percentage of germination increased by almost 20% compared to the untreated control), while the seed and seedling vigour was also positively affected. On the other hand, higher exposure times of CAPP generated in a nitrogen atmosphere significantly inhibited succinate dehydrogenase activity, but stimulated lactate and alcohol dehydrogenase activities, suggesting anoxygenic metabolism. It was also found that plasma exposure caused a slight increment in the level of primary DNA damage in ambient air- and oxygen-CAPP treatments, and more significant DNA damage was found in nitrogen-CAPP treatments. Although a higher level of DNA damage was also detected in the negative control (untreated seeds), this might be associated with the age of seeds followed by their lower germination capacity (with the germination percentage reaching only about 60%).

| S-EPMC7833387 | biostudies-literature

Plasma-Activated Hydrogels for Microbial Disinfection.

Project description:A continuous risk from microbial infections poses a major environmental and public health challenge. As an emerging strategy for inhibiting bacterial infections, plasma-activated water (PAW) has proved to be highly effective, environmental-friendly, and non-drug resistant to a broad range of microorganisms. However, the relatively short lifetime of reactive oxygen and nitrogen species (RONS) and the high spreadability of liquid PAW inevitably limit its real-life applications. In this study, plasma-activated hydrogel (PAH) is developed to act as reactive species carrier that allow good storage and controlled slow-release of RONS to achieve long-term antibacterial effects. Three hydrogel materials, including hydroxyethyl cellulose (HEC), carbomer 940 (Carbomer), and acryloyldimethylammonium taurate/VP copolymer (AVC) are selected, and their antibacterial performances under different plasma activation conditions are investigated. It is shown that the composition of the gels plays the key role in determining their biochemical functions after the plasma activation. The antimicrobial performance of AVC is much better than that of PAW and the other two hydrogels, along with the excellent stability to maintain the antimicrobial activity for more than 14 days. The revealed mechanism of the antibacterial ability of the PAH identifies the unique combination of short-lived species (1 O2 , ∙OH, ONOO- and O2 - ) stored in hydrogels. Overall, this study demonstrates the efficacy and reveals the mechanisms of the PAH as an effective and long-term disinfectant capable of delivering and preserving antibacterial chemistries for biomedical applications.

| S-EPMC10190649 | biostudies-literature

Cold radiofrequency plasma treatment modifies wettability and germination speed of plant seeds.

Project description:We report the possibility to modify the wetting properties of the surfaces of a diversity of seeds including: lentils (Lens culinaris), beans (Phaseolus vulgaris) and wheat (Triticum, species C9) by cold radiofrequency air plasma treatment. Air plasma treatment leads to the dramatic decrease in the apparent contact angle. Moreover, the speed of germination and yield (germination rate) of seeds can be modified by preliminary plasma treatment. The change in the wetting properties of seeds is at least partially due to oxidation of their surface under plasma treatment. Significant growth of the peaks corresponding to the nitrogen containing groups in the mass spectra of air plasma treated seeds was registered by TOF-SIMS spectroscopy.

| S-EPMC3473364 | biostudies-other

Inactivation Effect of Germination Combined with Cold Plasma Treatment on Bacillus licheniformis Spores

Project description: Not available

| S-EPMC10706397 | biostudies-literature

Enhancement of Wheat Seed Germination, Seedling Growth and Nutritional Properties of Wheat Plantlet Juice by Plasma Activated Water.

Project description:Previous studies have shown the great potential of using plasma-activated water (PAW) on improving agriculture seed germination, however, information on the influence of PAW on crop plantlet juice remains scanty. In this research, the effect of PAW generated by atmosphere pressure Ar-O2 plasma jet for 1-5 min on wheat seed germination, seedling growth and nutritional properties of wheat plantlet juice was investigated. Results revealed that all PAWs could enhance wheat seed germination and seedling growth in 7 days by improving the germination rate, germination index, fresh weight, dry weight and vigour index, and especially that PAW activated for 3 min (PAW-3) showed the best overall performance. In addition, the application of PAWs enhanced the nutritional properties of wheat plantlet juice from those grown for 14 days by improving total soluble solids, protein content, photosynthetic pigments, total phenolic content, antioxidant activity, enzyme activity, free amino acids and minerals content, and the best enhancement was also observed in PAW-3. It was concluded that PAWs would be an effective technique to enhance the growth and nutritional properties of crop sprouts, which could be served as functional foods in many forms.Supplementary informationThe online version contains supplementary material available at 10.1007/s00344-022-10677-3.

| S-EPMC9152647 | biostudies-literature

Transcriptomic analysis of seed germination improvement of Andrographis paniculata responding to air plasma treatment.

Project description:The plasma seed treatment is effective for promoting seed germination in many crops. However, the biological mechanism remains unclear. Therefore, mRNA sequencing was used to screen differentially expressed genes in the germination process of Andrographis paniculata seeds treated with air plasma (power density = 8.99 J/cm3). Following plasma treatment, the germination percentages were significantly higher than those of the control, they were 3.5±0.6% vs. 0 at 28 hours after sowing (HAS) and 50.3±2.6% vs. 37.3±1.7% at 48 HAS. After unigenes were assembled and annotated, 125 differentially expressed genes (DEGs) were detected at 28 HAS, compared with nine DEGs at 48 HAS, but no DEGs were detected at 0 HAS, indicating that air plasma treatment mainly changed the gene expression of A. paniculata seeds at 28 HAS. The NCED5 expression level of the treated group was less than one-fifth of the control, and the expressions of three ethylene response factors were significantly higher than the control at 28 HAS, indicating that lower abscisic acid levels play an important role and ethylene signal transduction also participates in radicle protrusion. ACO, NRT1 and PRP3 expressions were significantly higher than in the control at 48 HAS, suggesting that higher ethylene levels cause the endosperm cap to weaken and start to grow root hairs and lateral roots earlier. These findings reveal that plasma promotes seed germination mainly by regulating the expression of hormone-related genes. And the possible signal transduction of related hormones was discussed.

| S-EPMC7580921 | biostudies-literature

Improving Seed Morphology and Germination Potential in Australian Native Grasses Using Seed Enhancement Technologies.

Project description:Difficult to handle seed material and poor germination commonly limit the uptake of native grasses in restoration and commercial-scale seeding efforts. Seed enhancement technologies (SETs) offer valuable solutions for improving the handling of seed material and optimising germination. This study considered eight widespread Australian native grasses; two representative of Mediterranean to temperate climates ('cool-climate' species) and six representative of arid to subtropical climates ('warm-climate' species). Through a series of experiments, this study logically selected and applied SET treatments to improve seed handling and germination for each study species. Seed handling was prioritised and addressed using flash flaming and/or acid digestion, while hydropriming was used following seed-handling treatments to enhance germination. Flash flaming and acid digestion were both applied to successfully reduce or remove bulky floret structures while maintaining or improving germination. Flaming at 110 ± 10 °C with continuous exposure for 10 min and acid digestion concentrations of 75-80% with exposure times of 1-2.5 min were generally successful. Sub-optimal concentrations of sulphuric acid often compromised germination. Hydropriming did not improve germination outcomes when applied following flaming or acid digestion. Optimising SETs for germination, emergence and establishment in different environments, and the viability and costs of application on larger seed batches are key considerations for the implementation and upscaling of SETs in the future.

| S-EPMC10346785 | biostudies-literature

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data