Ontology highlight
ABSTRACT:
SUBMITTER: Eichenberger BT
PROVIDER: S-EPMC8287908 | biostudies-literature | 2021 Jul
REPOSITORIES: biostudies-literature
Eichenberger Bastian Th BT Zhan YinXiu Y Rempfler Markus M Giorgetti Luca L Chao Jeffrey A JA
Nucleic acids research 20210701 13
Detection of diffraction-limited spots in single-molecule microscopy images is traditionally performed with mathematical operators designed for idealized spots. This process requires manual tuning of parameters that is time-consuming and not always reliable. We have developed deepBlink, a neural network-based method to detect and localize spots automatically. We demonstrate that deepBlink outperforms other state-of-the-art methods across six publicly available datasets containing synthetic and e ...[more]