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DeepBlink: threshold-independent detection and localization of diffraction-limited spots.


ABSTRACT: Detection of diffraction-limited spots in single-molecule microscopy images is traditionally performed with mathematical operators designed for idealized spots. This process requires manual tuning of parameters that is time-consuming and not always reliable. We have developed deepBlink, a neural network-based method to detect and localize spots automatically. We demonstrate that deepBlink outperforms other state-of-the-art methods across six publicly available datasets containing synthetic and experimental data.

SUBMITTER: Eichenberger BT 

PROVIDER: S-EPMC8287908 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature

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deepBlink: threshold-independent detection and localization of diffraction-limited spots.

Eichenberger Bastian Th BT   Zhan YinXiu Y   Rempfler Markus M   Giorgetti Luca L   Chao Jeffrey A JA  

Nucleic acids research 20210701 13


Detection of diffraction-limited spots in single-molecule microscopy images is traditionally performed with mathematical operators designed for idealized spots. This process requires manual tuning of parameters that is time-consuming and not always reliable. We have developed deepBlink, a neural network-based method to detect and localize spots automatically. We demonstrate that deepBlink outperforms other state-of-the-art methods across six publicly available datasets containing synthetic and e  ...[more]

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