DeepBlink: threshold-independent detection and localization of diffraction-limited spots.

ABSTRACT: Detection of diffraction-limited spots in single-molecule microscopy images is traditionally performed with mathematical operators designed for idealized spots. This process requires manual tuning of parameters that is time-consuming and not always reliable. We have developed deepBlink, a neural network-based method to detect and localize spots automatically. We demonstrate that deepBlink outperforms other state-of-the-art methods across six publicly available datasets containing synthetic and experimental data.

SUBMITTER: Eichenberger BT 

PROVIDER: S-EPMC8287908 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature