Dataset Information

Understanding divergent domestication traits from the whole-genome sequencing of swamp- and river-buffalo populations.

ABSTRACT: Domesticated buffaloes have been integral to rice-paddy agro-ecosystems for millennia, yet relatively little is known about the buffalo genomics. Here, we sequenced and assembled reference genomes for both swamp and river buffaloes and we re-sequenced 230 individuals (132 swamp buffaloes and 98 river buffaloes) sampled from across Asia and Europe. Beyond the many actionable insights that our study revealed about the domestication, basic physiology and breeding of buffalo, we made the striking discovery that the divergent domestication traits between swamp and river buffaloes can be explained with recent selections of genes on social behavior, digestion metabolism, strengths and milk production.

SUBMITTER: Luo X

PROVIDER: S-EPMC8289072 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature

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Understanding divergent domestication traits from the whole-genome sequencing of swamp- and river-buffalo populations.

Luo Xier X Zhou Yu Y Zhang Bing B Zhang Yi Y Wang Xiaobo X Feng Tong T Li Zhipeng Z Cui Kuiqing K Wang Zhiqiang Z Luo Chan C Li Hui H Deng Yanfei Y Lu Fenghua F Han Jianlin J Miao Yongwang Y Mao Huaming H Yi Xiaoyan X Ai Cheng C Wu Shigang S Li Alun A Wu Zhichao Z Zhuo Zijun Z Da Giang Do D Mitra Bikash B Vahidi Mohammad Farhad MF Mansoor Shahid S Al-Bayatti Sahar Ahmed SA Sari Eka Meutia EM Gorkhali Neena Amatya NA Prastowo Sigit S Shafique Laiba L Ye Guoyou G Qian Qian Q Chen Baoshan B Shi Deshun D Ruan Jue J Liu Qingyou Q

National science review 20200217 3

Domesticated buffaloes have been integral to rice-paddy agro-ecosystems for millennia, yet relatively little is known about the buffalo genomics. Here, we sequenced and assembled reference genomes for both swamp and river buffaloes and we re-sequenced 230 individuals (132 swamp buffaloes and 98 river buffaloes) sampled from across Asia and Europe. Beyond the many actionable insights that our study revealed about the domestication, basic physiology and breeding of buffalo, we made the striking di ...[more]

PMID: 34692087

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Project description:The Chinese swamp buffalo (Bubalis bubalis) is vital to the lives of small farmers and has tremendous economic importance. However, a lack of genomic information has hampered research on augmenting marker assisted breeding programs in this species. Thus, a high-throughput transcriptomic sequencing of B. bubalis was conducted to generate transcriptomic sequence dataset for gene discovery and molecular marker development. Illumina paired-end sequencing generated a total of 54,109,173 raw reads. After trimming, de novo assembly was performed, which yielded 86,017 unigenes, with an average length of 972.41 bp, an N50 of 1,505 bp, and an average GC content of 49.92%. A total of 62,337 unigenes were successfully annotated. Among the annotated unigenes, 27,025 (43.35%) and 23,232 (37.27%) unigenes showed significant similarity to known proteins in NCBI non-redundant protein and Swiss-Prot databases (E-value < 1.0E-5), respectively. Of these annotated unigenes, 14,439 and 15,813 unigenes were assigned to the Gene Ontology (GO) categories and EuKaryotic Ortholog Group (KOG) cluster, respectively. In addition, a total of 14,167 unigenes were assigned to 331 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. Furthermore, 17,401 simple sequence repeats (SSRs) were identified as potential molecular markers. One hundred and fifteen primer pairs were randomly selected for amplification to detect polymorphisms. The results revealed that 110 primer pairs (95.65%) yielded PCR amplicons and 69 primer pairs (60.00%) presented polymorphisms in 35 individual buffaloes. A phylogenetic analysis showed that the five swamp buffalo populations were clustered together, whereas two river buffalo breeds clustered separately. In the present study, the Illumina RNA-seq technology was utilized to perform transcriptome analysis and SSR marker discovery in the swamp buffalo without using a reference genome. Our findings will enrich the current SSR markers resources and help spearhead molecular genetic research studies on the swamp buffalo.

Dataset Information

Understanding divergent domestication traits from the whole-genome sequencing of swamp- and river-buffalo populations.

Publications

Understanding divergent domestication traits from the whole-genome sequencing of swamp- and river-buffalo populations.

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