Browse
Submit Data
Databases
API
Help

Dataset Information

18 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Typical Fluorescent Sensors Exploiting Molecularly Imprinted Hydrogels for Environmentally and Medicinally Important Analytes Detection.

ABSTRACT: In this work, two typical fluorescent sensors were generated by exploiting molecularly imprinted polymeric hydrogels (MIPGs) for zearalenone (ZON) and glucuronic acid (GA) detection, via the analyte's self-fluorescence property and receptor's fluorescence effect, respectively. Though significant advances have been achieved on MIPG-fluorescent sensors endowed with superior stability over natural receptor-sensors, there is an increasing demand for developing sensing devices with cost-effective, easy-to-use, portable advantages in terms of commercialization. Zooming in on the commercial potential of MIPG-fluorescent sensors, the MIPG_ZON is synthesized using zearalanone (an analogue of ZON) as template, which exhibits good detection performance even in corn samples with a limit of detection of 1.6 μM. In parallel, fluorescein-incorporated MIPG_GA is obtained and directly used for cancer cell imaging, with significant specificity and selectivity. Last but not least, our consolidated application results unfold new opportunities for MIPG-fluorescent sensors for environmentally and medicinally important analytes detection.

SUBMITTER: Zou L

PROVIDER: S-EPMC8293044 | biostudies-literature |

REPOSITORIES: biostudies-literature

ACCESS DATA

Json Xml

Similar Datasets

Bovine Serum Albumin Molecularly Imprinted Electrochemical Sensors Modified by Carboxylated Multi-Walled Carbon Nanotubes/CaAlg Hydrogels.

Project description:In this paper, sodium alginate (NaAlg) was used as functional monomers, bovine serum albumin (BSA) was used as template molecules, and calcium chloride (CaCl2) aqueous solution was used as a cross-linking agent to prepare BSA molecularly imprinted carboxylated multi-wall carbon nanotubes (CMWCNT)/CaAlg hydrogel films (MIPs) and non-imprinted hydrogel films (NIPs). The adsorption capacity of the MIP film for BSA was 27.23 mg/g and the imprinting efficiency was 2.73. The MIP and NIP hydrogel film were loaded on the surface of the printed electrode, and electrochemical performance tests were carried out by electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV) using the electrochemical workstation. The loaded MIP film and NIP film effectively improved the electrochemical signal of the bare carbon electrode. When the pH value of the Tris HCl elution solution was 7.4, the elution time was 15 min and the adsorption time was 15 min, and the peak currents of MIP-modified electrodes and NIP-modified electrodes reached their maximum values. There was a specific interaction between MIP-modified electrodes and BSA, exhibiting specific recognition for BSA. In addition, the MIP-modified electrodes had good anti-interference, reusability, stability, and reproducibility. The detection limit (LOD) was 5.6 × 10-6 mg mL-1.

| S-EPMC10454541 | biostudies-literature

Molecularly Imprinted Polymer-Based Sensors for Priority Pollutants.

Project description:Globally, there is growing concern about the health risks of water and air pollution. The U.S. Environmental Protection Agency (EPA) has developed a list of priority pollutants containing 129 different chemical compounds. All of these chemicals are of significant interest due to their serious health and safety issues. Permanent exposure to some concentrations of these chemicals can cause severe and irrecoverable health effects, which can be easily prevented by their early identification. Molecularly imprinted polymers (MIPs) offer great potential for selective adsorption of chemicals from water and air samples. These selective artificial bio(mimetic) receptors are promising candidates for modification of sensors, especially disposable sensors, due to their low-cost, long-term stability, ease of engineering, simplicity of production and their applicability for a wide range of targets. Herein, innovative strategies used to develop MIP-based sensors for EPA priority pollutants will be reviewed.

| S-EPMC8037679 | biostudies-literature

A simple approach to prepare fluorescent molecularly imprinted nanoparticles.

Project description:Fluorescent molecularly imprinted polymers (FMIPs) are gaining increasing attention in analytical and medical sciences, particularly silica-based FMIPs due to their low cost, environmentally friendly nature and good biocompatibility. However, at present, silica-based FMIPs are usually prepared through several steps and displayed low selectivity. Here, a simple approach was utilized for preparing silica-based FMIP nanoparticles. The polymerization was initiated by 3-aminopropyltriethoxysilane (APTES), which also acted as the functional monomer in the imprinting system; in addition, to achieve one-pot synthesis, a fluorescent monomer was prepared by a simple reaction between fluorescein isothiocyanate (FITC) and APTES. The as-synthesized FMIP nanoparticles displayed high specificity and fast response time (<1 min) towards the target molecule. Environmental pH and buffer salt could affect the specific recognition behaviors of the FMIP nanoparticles. Such a simple catalyst-free synthetic technique could also be employed for the preparation of FMIP nanoparticles targeting other acidic molecules.

| S-EPMC8694954 | biostudies-literature

Polymer Composition Primarily Determines the Protein Recognition Characteristics of Molecularly Imprinted Hydrogels.

Project description:Synthetic hydrogels with the ability to recognize and bind target proteins are useful for a number of applications, including biosensing and therapeutic agent delivery. One popular method for fabricating recognitive hydrogels is molecular imprinting. A long-standing hypothesis of the field is that these molecularly imprinted polymers (MIPs) retain the chemical and geometric profile of their protein template, resulting in subsequent ability to recognize the template in solution. Here, we systematically determined the influence of network composition, as well as the identity, amount, and extraction of imprinting templates, on the protein binding of MIPs. Network composition (i.e. the relative number of ionizable and hydrophobic groups) explained the extent of protein adsorption in all cases. The identity and amount of imprinting template, albeit a protein or synthetic polymer (PEG) of similar molecular weight, did not significantly influence the amount of protein bound. While the purification method influenced the extent of template adsorption, it did so by chemically modifying the network (acrylamide hydrolysis, increasing the acid content by up to 21%) and not by voiding occupied MIP pores. Therefore, our results indicate that material composition determines the extent to which MIPs bind template and non-template proteins.

| S-EPMC7807727 | biostudies-literature

Bovine serum albumin detection by using molecularly imprinted surface plasmon resonance sensors.

Project description:Molecular imprinted polymers (MIP) have key-lock pattern binding properties specific to the size and shape of target molecules. In this study, we have prepared detection platforms based on a molecularly imprinted surface plasmon resonance (SPR) sensor that can detect bovine serum albumin (BSA) sensitively, selectively, quickly, and in real time. The polymeric film prepared on the SPR sensor surface by molecular imprinting method was obtained by selecting the N-methacryloyl-(L)-glutamic acid molecule as a suitable functional monomer using ultraviolet polymerization. Three different imprinting methods, such as epitope, bulk, and surface imprinting methods, were used to examine the imprinting efficiency. Real-time measurements were performed with BSA imprinted SPR sensor provide linearity in the concentration range from 0.10 to 7.50 nM and indicate a detection limit value of 0.015 nM. Furthermore, we performed the selectivity experiments, where transferrin and hemoglobin were chosen as competitor agents. Overall, the SPR sensor prepared by the epitope imprinting approach has been found to be highly selective and sensitive for bovine serum albumin. To statistically assess the reusability of the sensor, intraday experiments were tested three times with five replicates. The RSD% value less than <1.3 indicates high reproducibility for both sensor production and reproducibility of the method. Validation studies were carried out via enzyme-linked immunosorbent analysis technique (ELISA) in order to demonstrate the applicability of the BSA imprinted SPR sensor. Due to their features such as reusability, fast response time, and ease of use, these SPR sensors, which could be used as an alternative to albumin monitoring approaches, can also be adapted to detect and monitor other proteins in real time.

| S-EPMC10734745 | biostudies-literature

Molecularly imprinted polymer decorated nanoporous gold for highly selective and sensitive electrochemical sensors.

Project description:Electrochemical nanosensors based on nanoporous gold leaf (NPGL) and molecularly imprinted polymer (MIP) are developed for pharmaceutical analysis by using metronidazole (MNZ) as a model analyte. NPGL, serving as the loading platform for MIP immobilization, possesses large accessible surface area with superb electric conductivity, while electrochemically synthesized MIP thin layer affords selectivity for specific recognition of MNZ molecules. For MNZ determination, the hybrid electrode shows two dynamic linear range of 5 × 10(-11) to 1 × 10(-9) mol L(-1) and 1 × 10(-9) to 1.4 × 10(-6) mol L(-1) with a remarkably low detection limit of 1.8 × 10(-11) mol L(-1) (S/N = 3). In addition, the sensor exhibits high binding affinity and selectivity towards MNZ with excellent reproducibility and stability. Finally, the reliability of MIP-NPGL for MNZ detection is proved in real fish tissue samples, demonstrating the potential for the proposed electrochemical sensors in monitoring drug and biological samples.

| S-EPMC4287724 | biostudies-other

Molecularly Imprinted Micelles for Fluorescent Sensing of Nonsteroidal Anti-Inflammatory Drugs (NSAIDs).

Project description:Nonsteroidal anti-inflammatory drugs (NSAIDs) are widely used over-the-counter drugs and their uncontrolled disposal is a significant environmental concern. Although their fluorescent sensing is a desirable method of detection for its sensitivity and simplicity, the structural similarity of the drugs makes the design of selective sensors highly challenging. A thiourea-based fluorescent functional monomer was identified in this work to enable highly efficient synthesis of molecularly imprinted nanoparticle (MINP) sensors for NSAIDs such as Indomethacin or Tolmetin. Micromolar binding affinities were obtained in aqueous solution, with binding selectivities comparable to those reported for polyclonal antibodies. The detection limit was ~50 ng/mL in aqueous solution, and common carboxylic acids such as acetic acid, benzoic acid, and citric acid showed negligible interference.

| S-EPMC7945965 | biostudies-literature

Fluorescent molecularly imprinted polymer particles for glyphosate detection using phase transfer agents.

Project description:In this work, molecular imprinting was combined with direct fluorescence detection of the pesticide Glyphosate (GPS). Firstly, the solubility of highly polar GPS in organic solvents was improved by using lipophilic tetrabutylammonium (TBA+) and tetrahexylammonium (THA+) counterions. Secondly, to achieve fluorescence detection, a fluorescent crosslinker containing urea-binding motifs was used as a probe for GPS-TBA and GPS-THA salts in chloroform, generating stable complexes through hydrogen bond formation. The GPS/fluorescent dye complexes were imprinted into 2-3 nm fluorescent molecularly imprinted polymer (MIP) shells on the surface of sub-micron silica particles using chloroform as porogen. Thus, the MIP binding behavior could be easily evaluated by fluorescence titrations in suspension to monitor the spectral changes upon addition of the GPS analytes. While MIPs prepared with GPS-TBA and GPS-THA both displayed satisfactory imprinting following titration with the corresponding analytes in chloroform, GPS-THA MIPs displayed better selectivity against competing molecules. Moreover, the THA+ counterion was found to be a more powerful phase transfer agent than TBA+ in a biphasic assay, enabling the direct fluorescence detection and quantification of GPS in water. A limit of detection of 1.45 µM and a linear range of 5-55 µM were obtained, which match well with WHO guidelines for the acceptable daily intake of GPS in water (5.32 µM).

| S-EPMC9391380 | biostudies-literature

Selective detection of phospholipids using molecularly imprinted fluorescent sensory core-shell particles.

Project description:Sphingosine-1-phosphate (S1P) is a bioactive sphingo-lipid with a broad range of activities coupled to its role in G-protein coupled receptor signalling. Monitoring of both intra and extra cellular levels of this lipid is challenging due to its low abundance and lack of robust affinity assays or sensors. We here report on fluorescent sensory core-shell molecularly imprinted polymer (MIP) particles responsive to near physiologically relevant levels of S1P and the S1P receptor modulator fingolimod phosphate (FP) in spiked human serum samples. Imprinting was achieved using the tetrabutylammonium (TBA) salt of FP or phosphatidic acid (DPPA·Na) as templates in combination with a polymerizable nitrobenzoxadiazole (NBD)-urea monomer with the dual role of capturing the phospho-anion and signalling its presence. The monomers were grafted from ca 300 nm RAFT-modified silica core particles using ethyleneglycol dimethacrylate (EGDMA) as crosslinker resulting in 10-20 nm thick shells displaying selective fluorescence response to the targeted lipids S1P and DPPA in aqueous buffered media. Potential use of the sensory particles for monitoring S1P in serum was demonstrated on spiked serum samples, proving a linear range of 18-60 µM and a detection limit of 5.6 µM, a value in the same range as the plasma concentration of the biomarker.

| S-EPMC7303128 | biostudies-literature

Molecularly Imprinted Magnetic Fluorescent Nanocomposite-Based Sensor for Selective Detection of Lysozyme.

Project description:A new strategy for the design and construction of molecularly imprinted magnetic fluorescent nanocomposite-based-sensor is proposed. This multifunctional nanocomposite exhibits the necessary optics, magnetism and biocompatibility for use in the selective fluorescence detection of lysozyme. The magnetic fluorescent nanocomposites are prepared by combining carboxyl- functionalized Fe3O4 magnetic nanoparticles with l-cysteine-modified zinc sulfide quantum dots (MNP/QDs). Surface molecular imprinting technology was employed to coat the lysozyme molecularly imprinted polymer (MIP) layer on the MNP/QDs to form a core-shell structure. The molecularly imprinted MNP/QDs (MNP/QD@MIPs) can rapidly separate the target protein and then use fluorescence sensing to detect the protein; this reduces the background interference, and the selectivity and sensitivity of the detection are improved. The molecularly imprinted MNP/QDs sensor presented good linearity over a lysozyme concentration range from 0.2 to 2.0 μM and a detection limit of 4.53 × 10-3 μM for lysozyme. The imprinting factor of the MNP/QD@MIPs was 4.12, and the selectivity coefficient ranged from 3.19 to 3.85. Furthermore, the MNP/QD@MIPs sensor was applied to detect of lysozyme in human urine and egg white samples with recoveries of 95.40-103.33%. Experimental results showed that the prepared MNP/QD@MIPs has potential for selective magnetic separation and fluorescence sensing of target proteins in biological samples.

| S-EPMC8232576 | biostudies-literature

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data