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Preparation, Properties and Cell Biocompatibility of Room Temperature LCST-Hydrogels Based on Thermoresponsive PEO Stars.

ABSTRACT: A series of star and linear polymers based on a poly(ethylene oxide) core and poly(diethylene glycol ethyl ether acrylate) outer arms were synthesised by atom-transfer radical polymerization. The polydispersity of the polymers were low, showing good control of initiation and growth. They all showed lower critical solution (LCST) behaviour, and at 30% concentration most gelled at or below room temperature. The behaviour depended on the number and length of the arms, with the polymers with longer arms gelling at a lower temperature and producing stiffer gels. The shear modulus of the gels varied between 1 and 48 kPa, with the gelling temperature varying between 16 and 23 °C. Attempted cell cultures with the polymers proved unsuccessful, which was determined to be due to the high concentration of polymers needed for gelling.

SUBMITTER: Santoso B

PROVIDER: S-EPMC8293143 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature

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Preparation, Properties and Cell Biocompatibility of Room Temperature LCST-Hydrogels Based on Thermoresponsive PEO Stars.

Santoso Bagus B Turner Paul R PR Hanton Lyall R LR Moratti Stephen C SC

Gels (Basel, Switzerland) 20210706 3

A series of star and linear polymers based on a poly(ethylene oxide) core and poly(diethylene glycol ethyl ether acrylate) outer arms were synthesised by atom-transfer radical polymerization. The polydispersity of the polymers were low, showing good control of initiation and growth. They all showed lower critical solution (LCST) behaviour, and at 30% concentration most gelled at or below room temperature. The behaviour depended on the number and length of the arms, with the polymers with longer ...[more]

PMID: 34287296

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Project description:We report the development of thermoresponsive magnetic hydrogels based on poly(N-isopropylacrylamide) encapsulation of Fe3O4 magnetic nanostructures (MNS). In particular, we examined the effects of hydrogels encapsulated with poly-ethylene glycol (PEG) and polyhedral oligomeric silsesquioxane (POSS) surface modified Fe3O4 MNS on magnetic resonance (MR) T2 (transverse spin relaxation) contrast enhancement and associated delivery efficacy of absorbed therapeutic cargo. The microstructural characterization reveal the regular spherical shape and size (∼200 nm) of the hydrogels with elevated hydrophilic to hydrophobic transition temperature (∼40 °C) characterized by LCST (lower critical solution temperature) due to the presence of encapsulated MNS. The hydrogel-MNS (HGMNS) system encapsulated with PEG functionalized Fe3O4 of 12 nm size (HGMNS-PEG-12) exhibited relaxivity rate (r2) of 173 mM(-1) s(-1) compared to 129 mM(-1) s(-1) obtained for hydrogel-MNS system encapsulated with POSS functionalized Fe3O4 (HGMNS-POSS-12) of the same size. Further studies with HGMNS-PEG-12 with absorbed drug doxorubicin (DOX) reveals approximately two-fold enhance in release during 1 h RF (radio-frequency) field exposure followed by 24 h incubation at 37 °C. Quantitatively, it is 2.1 μg mg(-1) (DOX/HGMNS) DOX release with RF exposure while only 0.9 μg mg(-1) release without RF exposure for the same period of incubation. Such enhanced release of therapeutic cargo is attributed to micro-environmental heating in the surroundings of MNS as well as magneto-mechanical vibrations under high frequency RF inside hydrogels. Similarly, RF-induced in vitro localized drug delivery studies with HeLa cell lines for HGMNS-PEG-12 resulted in more than 80% cell death with RF field exposures for 1 h. We therefore believe that magnetic hydrogel system has in vivo theranostic potential given high MR contrast enhancement from encapsulated MNS and RF-induced localized therapeutic delivery in one nanoconstruct.

Dataset Information

Preparation, Properties and Cell Biocompatibility of Room Temperature LCST-Hydrogels Based on Thermoresponsive PEO Stars.

Publications

Preparation, Properties and Cell Biocompatibility of Room Temperature LCST-Hydrogels Based on Thermoresponsive PEO Stars.

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