Impact of microRNA-210 on wound healing among the patients with diabetic foot ulcer.

ABSTRACT:

Aim

Diabetic foot ulcer (DFU) is a major concern in diabetes and its control requires in-depth molecular investigation. The present study aimed to screen the expression of microRNA-210 (miR-210) and its association in hypoxic pathway in DFU patients.

Methods

The study consists of 3 groups of circulation samples (50 in each group of: healthy volunteers, T2DM and T2DM with DFU) and 2 groups of tissue samples (10 in each group of: control and T2DM with DFU). Expression of miR-210 and hypoxia inducible factor-1 alpha (HIF-1α), and its responsive genes such as VEGF, TNF-α, IL-6, BCl2, Bax and Caspase 3 were analyzed by RT-PCR, Western blot and ELISA analyses.

Results

The HIF-1α expression decreased in DFU patients with increased miR-210 expression in both circulation and t

SUBMITTER: Pichu S 

PROVIDER: S-EPMC8297780 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature