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Estimating the effect of tracking tag weight on insect movement using video analysis: A case study with a flightless orthopteran.

ABSTRACT: In this study, we describe an inexpensive and rapid method of using video analysis and identity tracking to measure the effects of tag weight on insect movement. In a laboratory experiment, we assessed the tag weight and associated context-dependent effects on movement, choosing temperature as a factor known to affect insect movement and behavior. We recorded the movements of groups of flightless adult crickets Gryllus locorojo (Orthoptera:Gryllidae) as affected by no tag (control); by light, medium, or heavy tags (198.7, 549.2, and 758.6 mg, respectively); and by low, intermediate, or high temperatures (19.5, 24.0, and 28.3°C, respectively). Each individual in each group was weighed before recording and was recorded for 3 consecutive days. The mean (± SD) tag mass expressed as a percentage of body mass before the first recording was 26.8 ± 3.7% with light tags, 72 ± 11.2% with medium tags, and 101.9 ± 13.5% with heavy tags. We found that the influence of tag weight strongly depended on temperature, and that the negative effects on movement generally increased with tag weight. At the low temperature, nearly all movement properties were negatively influenced. At the intermediate and high temperatures, the light and medium tags did not affect any of the movement properties. The continuous 3-day tag load reduced the average movement speed only for crickets with heavy tags. Based on our results, we recommend that researchers consider or investigate the possible effects of tags before conducting any experiment with tags in order to avoid obtaining biased results.

SUBMITTER: Kalab O

PROVIDER: S-EPMC8297838 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Project description:Quantifying and understanding movement is critical for a wide range of questions in basic and applied ecology. Movement ecology is also fostered by technological advances that allow automated tracking for a wide range of animal species. However, for aquatic macroinvertebrates, such detailed methods do not yet exist. We developed a video tracking method for two different species of benthic macroinvertebrates, the crawling isopod Asellus aquaticus and the swimming fresh water amphipod Gammarus pulex. We tested the effects of different light sources and marking techniques on their movement behavior to establish the possibilities and limitations of the experimental protocol and to ensure that the basic handling of test specimens would not bias conclusions drawn from movement path analyses. To demonstrate the versatility of our method, we studied the influence of varying population densities on different movement parameters related to resting behavior, directionality, and step lengths. We found that our method allows studying species with different modes of dispersal and under different conditions. For example, we found that gammarids spend more time moving at higher population densities, while asellids rest more under similar conditions. At the same time, in response to higher densities, gammarids mostly decreased average step lengths, whereas asellids did not. Gammarids, however, were also more sensitive to general handling and marking than asellids. Our protocol for marking and video tracking can be easily adopted for other species of aquatic macroinvertebrates or testing conditions, for example, presence or absence of food sources, shelter, or predator cues. Nevertheless, limitations with regard to the marking protocol, material, and a species' physical build need to be considered and tested before a wider application, particularly for swimming species. Data obtained with this approach can deepen the understanding of population dynamics on larger spatial scales and of the effects of different management strategies on a species' dispersal potential.

Project description:The present study aimed to validate and compare the football-specific measurement accuracy of two optical tracking systems engineered by TRACAB. The "Gen4" system consists of two multi-camera units (a stereo pair) in two locations either side of the halfway line, whereas the distributed "Gen5" system combines two stereo pairs on each side of the field as well as two monocular systems behind the goal areas. Data were collected from 20 male football players in two different exercises (a football sport-specific running course and small-sided games) in a professional football stadium. For evaluating the accuracy of the systems, measures were compared against simultaneously recorded measures of a reference system (VICON motion capture system). Statistical analysis uses RMSE for kinematic variables (position, speed and acceleration) and the difference in percentages for performance indicators (e.g. distance covered, peak speed) per run compared to the reference system. Frames in which players were obviously not tracked were excluded. Gen5 had marginally better accuracy (0.08 m RMSE) for position measurements than Gen4 (0.09 m RMSE) compared to the reference. Accuracy difference in instantaneous speed (Gen4: 0.09 m?s-1 RMSE; Gen5: 0.08 m?s-1 RMSE) and acceleration (Gen4: 0.26 m?s-2 RMSE; Gen5: 0.21 m?s-2 RMSE) measurements were significant, but also trivial in terms of the effect size. For total distance travelled, both Gen4 (0.42 ± 0.60%) and Gen5 (0.27 ± 0.35%) showed only trivial deviations compared to the reference. Gen4 showed moderate differences in the low-speed distance travelled category (-19.41 ± 13.24%) and small differences in the high-speed distance travelled category (8.94 ± 9.49%). Differences in peak speed, acceleration and deceleration were trivial (<0.5%) for both Gen4 and Gen5. These findings suggest that Gen5's distributed camera architecture has minor benefits over Gen4's single-view camera architecture in terms of accuracy. We assume that the main benefit of the Gen5 towards Gen4 lies in increased robustness of the tracking when it comes to optical overlapping of players. Since differences towards the reference system were very low, both TRACAB's tracking systems can be considered as valid technologies for football-specific performance analyses in the settings tested as long as players are tracked correctly.

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Estimating the effect of tracking tag weight on insect movement using video analysis: A case study with a flightless orthopteran.

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