Unknown

Dataset Information

0

An on-demand, drop-on-drop method for studying enzyme catalysis by serial crystallography.


ABSTRACT: Serial femtosecond crystallography has opened up many new opportunities in structural biology. In recent years, several approaches employing light-inducible systems have emerged to enable time-resolved experiments that reveal protein dynamics at high atomic and temporal resolutions. However, very few enzymes are light-dependent, whereas macromolecules requiring ligand diffusion into an active site are ubiquitous. In this work we present a drop-on-drop sample delivery system that enables the study of enzyme-catalyzed reactions in microcrystal slurries. The system delivers ligand solutions in bursts of multiple picoliter-sized drops on top of a larger crystal-containing drop inducing turbulent mixing and transports the mixture to the X-ray interaction region with temporal resolution. We demonstrate mixing using fluorescent dyes, numerical simulations and time-resolved serial femtosecond crystallography, which show rapid ligand diffusion through microdroplets. The drop-on-drop method has the potential to be widely applicable to serial crystallography studies, particularly of enzyme reactions with small molecule substrates.

SUBMITTER: Butryn A 

PROVIDER: S-EPMC8298390 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC4920001 | biostudies-literature
| S-EPMC4937782 | biostudies-literature
| S-EPMC4052866 | biostudies-literature
| S-EPMC5376230 | biostudies-literature
| S-EPMC6168542 | biostudies-literature
| S-EPMC5977757 | biostudies-literature
| S-EPMC6926069 | biostudies-literature
| S-EPMC5668288 | biostudies-literature
| S-EPMC4566134 | biostudies-literature
| S-EPMC10971408 | biostudies-literature