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Rhamnolipids Nano-Micelles as a Potential Hand Sanitizer.

ABSTRACT: COVID-19 is a pandemic disease caused by the SARS-CoV-2, which continues to cause global health and economic problems since emerging in China in late 2019. Until now, there are no standard antiviral treatments. Thus, several strategies were adopted to minimize virus transmission, such as social distancing, face covering protection and hand hygiene. Rhamnolipids are glycolipids produced formally by Pseudomonas aeruginosa and as biosurfactants, they were shown to have broad antimicrobial activity. In this study, we investigated the antimicrobial activity of rhamnolipids against selected multidrug resistant bacteria and SARS-CoV-2. Rhamnolipids were produced by growing Pseudomonas aeruginosa strain LeS3 in a new medium formulated from chicken carcass soup. The isolated rhamnolipids were characterized for their molecular composition, formulated into nano-micelles, and the antibacterial activity of the nano-micelles was demonstrated in vitro against both Gram-negative and Gram-positive drug resistant bacteria. In silico studies docking rhamnolipids to structural and non-structural proteins of SARS-CoV-2 was also performed. We demonstrated the efficient and specific interaction of rhamnolipids with the active sites of these proteins. Additionally, the computational studies suggested that rhamnolipids have membrane permeability activity. Thus, the obtained results indicate that SARS-CoV-2 could be another target of rhamnolipids and could find utility in the fight against COVID-19, a future perspective to be considered.

SUBMITTER: Bakkar MR

PROVIDER: S-EPMC8300634 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Project description:Hospital-acquired infections (HAIs) are considered to be a major global healthcare challenge, in large part because of the development of microbial resistance to currently approved antimicrobial drugs. HAIs are frequently preventable through infection prevention and control measures, with hand hygiene as a key activity. Improving hand hygiene was reported to reduce the transmission of healthcare-associated pathogens and HAIs. Alcohol-based hand sanitizers are commonly used due to their rapid action and broad spectrum of microbicidal activity, offering protection against bacteria and viruses. However, their frequent administration has been reported to be associated with many side effects, such as skin sensitivity, skin drying, and cracks, which promote further skin infections. Thus, there is an essential need to find alternative approaches to hand sanitation. Rhamnolipids are glycolipids produced by Pseudomonas aeruginosa, and were shown to have broad antimicrobial activity as biosurfactants. We have previously demonstrated the antimicrobial activity of rhamnolipid nano-micelles against selected drug-resistant Gram-negative (Salmonella Montevideo and Salmonella Typhimurium) and Gram-positive bacteria (Staphylococcus aureus, Streptococcus pneumoniae). To the best of our knowledge, the antimicrobial activity of rhamnolipid nano-micelles in comparison to alcohol-based hand sanitizers against microorganisms commonly causing HAIs in Egypt-such as Acinetobacter baumannii and Staphylococcus aureus-has not yet been studied. In the present work, a comparative study of the antibacterial activity of rhamnolipid nano-micelles versus alcohol-based hand sanitizers was performed, and their safety profiles were also assessed. It was demonstrated that rhamnolipid nano-micelles had a comparable antibacterial activity to alcohol-based hand sanitizer, with a better safety profile, i.e., rhamnolipid nano-micelles are unlikely to cause any harmful effects on the skin. Thus, rhamnolipid nano-micelles could be recommended to replace alcohol-based hand sanitizers; however, they must still be tested by healthcare workers in healthcare settings to ascertain their antimicrobial activity and safety.

Project description:Earth's plastic pollution has increased due to the COVID-19 pandemic, and the world is on the doorstep of an enormous waste pandemic. The extensive use of mandatory personal protectives like masks, gloves, and PPE kits and the lack of proper waste management systems lead to a rise in the plastic pollution content of the earth. Such disposable and non-biodegradable personal protectives are thrown out to the environment after use. These distributed wastes pollute land, soil, and water bodies and effects their ecosystems. This research work establishes the concept of a waste-to-energy conversion approach to reuse COVID-19 scraps for green and sustainable development. Three-layered surgical masks and nitrile gloves were reused in this work after sterilization for energy harvesting and sensing applications by fabricating a 3D-printed contact-separation-based triboelectric nanogenerator. A piece of three-layered mask and nitrile gloves were placed inside the 3D structure as the top negative and bottom positive triboelectric materials with copper and aluminum as corresponding electrodes (MG-CS TENG). It can convert external mechanical motions into electrical energy. The maximum voltage, current, and power density obtained from the device are 50.7 V, 4.8 µA, and 6.39 µW/cm2, respectively, for a mechanical force of 9 N. The harvested energy was sufficient to power small-scale electronic devices like digital tally counters, wristwatches, lumex displays, and series connected 25 LEDs. MG-CS TENG was also performed as a pedal-operated touch sensor to dispense hand sanitizer. MG-CS TENG was pedal pressed to trigger a microcontroller and control the solenoid valve's opening and closing to regulate sanitizer flow. The setup was integrated using the internet of things (IoT) and Blynk cloud services for the remote monitoring and controlling of the sanitizer dispenser using a smartphone. This work contributes a substantial role in disaster management to suppress microplastic environmental pollution by reusing pandemic wastes for energy harvesting and sensing applications and preventing the spread of coronavirus through proper hand sanitization.

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Rhamnolipids Nano-Micelles as a Potential Hand Sanitizer.

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