VMP1, a novel prognostic biomarker, contributes to glioma development by regulating autophagy.

ABSTRACT:

Background

Malignant glioma, especially glioblastoma, is a highly aggressive disease with a dismal prognosis. Vacuole membrane protein 1 (VMP1) is a critical autophagy-associated protein with roles in oncogenesis and tumor progression. However, the contribution of VMP1 to glioma development as well as its prognostic value has not been established.

Methods

The expression of VMP1 and clinicopathologic data for 1996 glioma samples were collected from authoritative public databases to explore its prognostic value. Lentiviral CRISPR-Cas9 gene editing system was performed to deplete VMP1 expression. Apoptosis assays, cell cycle assays, colony formation assays, and EdU incorporation analysis were conducted to validate the biological function of VMP1. Transmission electron microscop

SUBMITTER: Lin W 

PROVIDER: S-EPMC8311950 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature