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Unified platform for genetic and serological detection of COVID-19 with single-molecule technology.

ABSTRACT: The COVID-19 pandemic raises the need for diverse diagnostic approaches to rapidly detect different stages of viral infection. The flexible and quantitative nature of single-molecule imaging technology renders it optimal for development of new diagnostic tools. Here we present a proof-of-concept for a single-molecule based, enzyme-free assay for detection of SARS-CoV-2. The unified platform we developed allows direct detection of the viral genetic material from patients' samples, as well as their immune response consisting of IgG and IgM antibodies. Thus, it establishes a platform for diagnostics of COVID-19, which could also be adjusted to diagnose additional pathogens.

SUBMITTER: Furth N

PROVIDER: S-EPMC8312974 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature

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Unified platform for genetic and serological detection of COVID-19 with single-molecule technology.

Furth Noa N Shilo Shay S Cohen Niv N Erez Nir N Fedyuk Vadim V Schrager Alexander M AM Weinberger Adina A Dror Amiel A AA Zigron Asaf A Shehadeh Mona M Sela Eyal E Srouji Samer S Amit Sharon S Levy Itzchak I Segal Eran E Dahan Rony R Jones Dan D Douek Daniel C DC Shema Efrat E

PloS one 20210726 7

The COVID-19 pandemic raises the need for diverse diagnostic approaches to rapidly detect different stages of viral infection. The flexible and quantitative nature of single-molecule imaging technology renders it optimal for development of new diagnostic tools. Here we present a proof-of-concept for a single-molecule based, enzyme-free assay for detection of SARS-CoV-2. The unified platform we developed allows direct detection of the viral genetic material from patients' samples, as well as thei ...[more]

PMID: 34310620

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Unified platform for genetic and serological detection of COVID-19 with single-molecule technology.

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An Integrated Platform for Serological Detection and Vaccination of COVID-19.

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Project description: Not available

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Project description:BackgroundSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection spreaded rapidly worldwide, as far as it has become a global pandemic. Therefore, the introduction of serological tests for determination of IgM and IgG antibodies has become the main diagnostic tool, useful for tracking the spread of the virus and for consequently allowing its containment. In our study we compared point of care test (POCT) lateral flow immunoassay (FIA) vs automated chemiluminescent immunoassay (CLIA), in order to assess their specificity and sensibility for COVID-19 antibodies detection.ResultsWe find that different specificities and sensitivities for IgM and IgG tests. Notably IgM POCT FIA method vs CLIA method (gold standard) has a low sensitivity (0.526), while IgG POCT FIA method vs CLIA method (gold standard) test has a much higher sensitivity (0.937); further, with respect of IgG, FIA and CLIA could arguably provide equivalent information.ConclusionsFIA method could be helpful in assessing in short time, the possible contagiousness of subjects that for work reasons cannot guarantee "social distancing".

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