Characterization of genomic DNA sequence of the candidate gene for FB_Mfu10 associated with fire blight resistance in Malus species.

ABSTRACT:

Objective

The proposed candidate gene underlying the Malus fusca fire blight resistance locus on chromosome 10 was previously predicted to possess 880 amino acids and 8 exons. Eight base pair (8 bp) insertion/deletion in the first exon potentially distinguished resistant genotypes from susceptible ones. This study aimed at analyzing the candidate gene sequence in another set of original resistant and susceptible progeny, characterizing the sequence in a transgenic line transformed with the candidate gene under its own native promoter, as well as deciphering the potential genomic differences between this candidate gene and its homolog in the 'Golden Delicious' doubled haploid genome (GDDH13).

Results

Sequences of amplicons of part of the candidate gene amplified in two progen

SUBMITTER: Emeriewen OF 

PROVIDER: S-EPMC8314441 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature