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Negative Impact of Pseudomonas aeruginosa Y12 on Its Host Musca domestica.

ABSTRACT: High concentrations of Pseudomonas aeruginosa Y12 significantly inhibit the development of housefly larvae and accelerate larvae death. In this study, the dynamic distribution of the gut microbiota of housefly larvae fed different concentrations of P. aeruginosa Y12 was investigated. Compared with low-concentration P. aeruginosa diets, orally administered high-concentration P. aeruginosa diets caused higher mortality and had a greater impact on the community structure and interaction network of intestinal flora in housefly larvae. The bacterial community of the gut microbiota in housefly larvae was reconstructed in 4 days. Bacterial abundance and diversity were significantly reduced in housefly larvae fed high concentrations of P. aeruginosa. With the growth of larvae, the relative abundances of Providencia, Proteus, Myroides, Klebsiella, and Alcaligenes increased significantly in housefly larvae fed with high concentrations of P. aeruginosa, while the relative abundances of Bordetella, Enterobacter, Morganella, Ochrobactrum, Alcaligenaceae, and Empedobacter were significantly reduced. To analyze the role of the gut microorganisms played on housefly development, a total of 10 cultivable bacterial species belonging to 9 genera were isolated from the intestine of housefly larvae among which Enterobacter hormaechei, Klebsiella pneumoniae, Enterobacter cloacae, Lysinibacillus fusiformis, and Bacillus safensis promoted the growth of larvae through feeding experiments. This study is the first to analyze the influence of high concentrations of P. aeruginosa on the gut microbiota of houseflies. Our study provides a basis for exploring the pathogenic mechanism of high concentrations of P. aeruginosa Y12 in houseflies.

SUBMITTER: Zhang Q

PROVIDER: S-EPMC8317488 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Project description:House flies are of major concern as vectors of food-borne pathogens to food crops. House flies are common pests on cattle feedlots and dairies, where they develop in and feed on animal waste. By contacting animal waste, house flies can acquire human pathogenic bacteria such as Escherichia coli and Salmonella spp., in addition to other bacteria, viruses, or parasites that may infect humans and animals. The subsequent dispersal of house flies from animal facilities to nearby agricultural fields containing food crops may lead to pre-harvest food contamination with these pathogens. We hypothesized that odors from honeydew, the sugary excreta produced by sucking insects feeding on crops, or molds and fungi growing on honeydew, may attract house flies, thereby increasing the risk of food crop contamination. House fly attraction to honeydew-contaminated plant material was evaluated using a laboratory bioassay. House flies were attracted to the following plant-pest-honeydew combinations: citrus mealybug on squash fruit, pea aphid on faba bean plants, whitefly on navel orange and grapefruit leaves, and combined citrus mealybug and cottony cushion scale on mandarin orange leaves. House flies were not attracted to field-collected samples of lerp psyllids on eucalyptus plants or aphids on crepe myrtle leaves. Fungi associated with field-collected honeydews were isolated and identified for further study as possible emitters of volatiles attractive to house flies. Two fungal species, Aureobasidium pullulans and Cladosporium cladosporioides, were repeatedly isolated from field-collected honeydew samples. Both fungal species were grown in potato dextrose enrichment broth and house fly attraction to volatiles from these fungal cultures was evaluated. House flies were attracted to odors from A. pullulans cultures but not to those of C. cladosporioides. Identification of specific honeydew odors that are attractive to house flies could be valuable for the development of improved house fly baits for management of this pest species.

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Negative Impact of Pseudomonas aeruginosa Y12 on Its Host Musca domestica.

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