Enforcing ATP hydrolysis enhanced anaerobic glycolysis and promoted solvent production in Clostridium acetobutylicum.

ABSTRACT:

Background

The intracellular ATP level is an indicator of cellular energy state and plays a critical role in regulating cellular metabolism. Depletion of intracellular ATP in (facultative) aerobes can enhance glycolysis, thereby promoting end product formation. In the present study, we examined this s trategy in anaerobic ABE (acetone-butanol-ethanol) fermentation using Clostridium acetobutylicum DSM 1731.

Results

Following overexpression of atpAGD encoding the subunits of water-soluble, ATP-hydrolyzing F₁-ATPase, the intracellular ATP level of 1731(pITF₁) was significantly reduced compared to control 1731(pIMP1) over the entire batch fermentation. The glucose uptake was markedly enhanced, achieving a 78.8% increase of volumetric glucose utilization rat

SUBMITTER: Dai Z 

PROVIDER: S-EPMC8320212 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature