SARS-CoV-2 antigen exposure history shapes phenotypes and specificity of memory CD8 T cells.

ABSTRACT: Although mRNA vaccine efficacy against severe COVID-19 remains high, variant emergence and breakthrough infections have changed vaccine policy to include booster immunizations. However, the effect of diverse and repeated antigen exposures on SARS-CoV-2 memory T cells is poorly understood. Here, we utilize DNA-barcoded MHC-multimers combined with scRNAseq and scTCRseq to capture the ex vivo profile of SARS-CoV-2-responsive T cells within a cohort of individuals with one, two, or three antigen exposures, including vaccination, primary infection, and breakthrough infection. We found that the order of exposure determined the relative distribution between spike- and non-spike-specific responses, with vaccination after infection leading to further expansion of spike-specific T cells and d

SUBMITTER: Minervina AA 

PROVIDER: S-EPMC8328067 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature