Unknown

Dataset Information

0

MiR-665 inhibits epithelial-to-mesenchymal transition in bladder cancer via the SMAD3/SNAIL axis.

ABSTRACT: Emerging research indicates that miRNAs can regulate cancer progression by influencing molecular pathways. Here, we studied miR-665, part of the DLK1-DIO3 miRNA cluster, which is downregulated by upstream methylation in bladder cancer. MiR-665 overexpression significantly downregulated the expression of SMAD3, phospho-SMAD3, and SNAIL, reversed epithelial-mesenchymal transition progression, and inhibited the migration of bladder cancer cells. To predict potential targets of miR-665, we used online databases and subsequently determined that miR-665 binds directly to the 3' untranslated region of SMAD3. Moreover, silencing of SMAD3 with small interfering RNAs phenocopied the effect of miR-665 overexpression, and overexpression of SMAD3 restored miR-665-overexpression-induced metastasis. This study revealed the role of the miR-665/SMAD3/SNAIL axis in bladder cancer, as well as the potential of miR-665 as a promising therapeutic target.

SUBMITTER: Wang W 

PROVIDER: S-EPMC8331018 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature

Json Xml
altmetric image

Publications

miR-665 inhibits epithelial-to-mesenchymal transition in bladder cancer via the SMAD3/SNAIL axis.

Wang Weiyu W   Ying Yufan Y   Xie Haiyun H   Li Jiangfeng J   Ma Xueyou X   He Liujia L   Xu Mingjie M   Chen Shiming S   Shen Haixiang H   Zheng Xiangyi X   Liu Ben B   Wang Xiao X   Xie Liping L  

Cell cycle (Georgetown, Tex.) 20210701 13

Emerging research indicates that miRNAs can regulate cancer progression by influencing molecular pathways. Here, we studied miR-665, part of the DLK1-DIO3 miRNA cluster, which is downregulated by upstream methylation in bladder cancer. MiR-665 overexpression significantly downregulated the expression of SMAD3, phospho-SMAD3, and SNAIL, reversed epithelial-mesenchymal transition progression, and inhibited the migration of bladder cancer cells. To predict potential targets of miR-665, we used onli  ...[more]

Similar Datasets

Unknown MET/SMAD3/SNAIL circuit mediated by miR-323a-3p is involved in regulating epithelial-mesenchymal transition progression in bladder cancer.
| S-EPMC5596538 | biostudies-literature
Unknown MiR-363 inhibits cisplatin chemoresistance of epithelial ovarian cancer by regulating snail-induced epithelial-mesenchymal transition.
| S-EPMC6177509 | biostudies-literature
Unknown MiR-22 suppresses epithelial-mesenchymal transition in bladder cancer by inhibiting Snail and MAPK1/Slug/vimentin feedback loop.
| S-EPMC5833802 | biostudies-literature
Unknown Circ_0000658 knockdown inhibits epithelial-mesenchymal transition in bladder cancer via miR-498-induced HMGA2 downregulation.
| S-EPMC8759287 | biostudies-literature
Unknown Lamp2 inhibits epithelial-mesenchymal transition by suppressing Snail expression in HCC.
| S-EPMC6084387 | biostudies-literature
Unknown MiR-135-5p inhibits TGF-β-induced epithelial-mesenchymal transition and metastasis by targeting SMAD3 in breast cancer.
| S-EPMC7532519 | biostudies-literature
Unknown WISP-1 Promotes Epithelial-Mesenchymal Transition in Oral Squamous Cell Carcinoma Cells Via the miR-153-3p/Snail Axis.
| S-EPMC6966565 | biostudies-literature
Unknown The VIM-AS1/miR-655/ZEB1 axis modulates bladder cancer cell metastasis by regulating epithelial-mesenchymal transition.
| S-EPMC8074428 | biostudies-literature
Unknown Blocking MIR155HG/miR-155 axis inhibits mesenchymal transition in glioma.
| S-EPMC5570208 | biostudies-literature
Unknown Glucose-6-phosphate dehydrogenase is indispensable in embryonic development by modulation of epithelial-mesenchymal transition via the NOX/Smad3/miR-200b axis.
| S-EPMC5849038 | biostudies-literature