Ultra-fast cycling for multiplexed cellular fluorescence imaging.
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ABSTRACT: Rapid analysis of single and scant cell populations is essential in modern diagnostics, yet existing methods are often limited and slow. Here we describe an ultra-fast, highly efficient cycling method for the analysis of single cells based on unique linkers for tetrazine (Tz) / trans-cyclooctene (TCO) mediated quenching. Surprisingly, the quenching reaction rates were more than 3 orders of magnitude faster (t1/2 < 1 sec) than predicted. This allowed multi-cycle staining and immune cell profiling within an hour, leveraging the accelerated kinetics to open new diagnostic possibilities for rapid cellular analyses.
SUBMITTER: Ko J
PROVIDER: S-EPMC8340598 | biostudies-literature |
REPOSITORIES: biostudies-literature
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